Is there anybody working in NIPT testing by NGS (Gene studio S5)?

we are planning to buy the gene studio S5 for starting the BIPT testing. we asked and inquired many times for knowing the cost per test for Nipt on above said instrument. i wish to know that what...

03 April 2019 581 0 View

Anybody can suggest a good Giemsa trypsin banding protocol ?

I am using SSC buffer to make a giemsa (20mg per 50 ml SSC buffer). we use aged slide (65 degree for overnight) for treating with Trypsin Solution for 20 sec at 20 degree. We get minor banding...

07 August 2018 4,400 4 View

PGMY11 primer set doesn't give any band with DNA sample which are shown positive with GP5+ and GP6+ primer set of cervical brush sample???

i screened many DNA samples extracted from the cervical brush sample for presence or absence of HPV DNA with primer set GP5+ /6+ and i got five positive sample. then i used that 5 positive samples...

07 August 2017 6,354 2 View

Any one have idea regarding sequence of Primers for Tuberculosis PCR?

There are many primers available in paper published, but most of all have problems with Tm, product size, nonspecific binding and many more. I need a best primer sequences for TB PCR. Is there...

02 March 2017 254 2 View

RAPD analysis of binomial matrix and dendogram preparation?

I have two variety, one is A - in vivo plant leaf and B - in vitro plant leaf. i had extracted DNA from these both samples A & B. Done PCR with 31 RAPD primers and got varies band pattern on...

08 September 2016 7,948 6 View

16s r-DNA PCR product giving three bands on gel, so which one should be considered?

I have Extracted DNA from six different actinomycetes bacterial strains and then do PCR with 27F & 1492R primers. i should get the band with size between 1400 to 1500 bp. but here i got three...

07 August 2016 9,941 6 View

RAPD analysis for the in-vitro and in-vivo plant samples of the same plant species?

I have done RAPD Analysis for one plant with its Invivo tissue and in vitro tissue samples. I have used 31 primer sets, so now i dont getting how much maximum variation can be considered for this...

07 August 2016 825 5 View

What should be the concentration and content of all PCR components for doing RAPD analysis?

i am going to do RAPD analysis of two different plant species with 31 different RAPD primers. I need to know the best suitable concentration and volume of primer, MgCl2, Taq pol, PCR buffer, and...

05 June 2016 914 3 View

Which HPLC method, column type and solvent system will best suite for Alginate analysis extracted from different bacterial strains?

05 June 2016 9,627 3 View

How can I remove a high polysaccharide content from DNA extracted with plant leaf?

I have extracted plant DNA from leaf sample. i got good precipitates when precipitated with ethanol and iso propanol. but when i dissolve the dried pellet in TE or molecular grade water, it became...

11 December 2015 4,297 4 View

What is your perception on using visual analysis or quantitative approaches to evaluate data from single-case research designs (n of 1 trials)?

Results of single-case research designs (i.e., n-of-1 trials) are often evaluated by visually inspecting the time-series graph and computing quantitative indices. A question our research team is...

03 March 2021 687 1 View

How do I increase the yield of my PCR product?

Hello, We would like to increase the yield of our PCR product. We are running a series of PCR reactions that is targeting ~1.1kb sequence. We begin each reaction with ~400pg of template DNA...

02 March 2021 4,029 3 View

Arena simulation - how do i model the following model in ARENA (see description) (new to ARENA)?

Hello, I'm fairly new to ARENA and I have the following problem: I have two queues in which, in each queue, a different order type arrives (both having an arrival rate of exponential 10). Order...

02 March 2021 2,431 3 View

Which is the most convenient manufacturing software for Additive Manufacturing?

Which tools can be used to generate machine codes for additive manufacturing?

02 March 2021 7,220 2 View

Research on Supply Chain Management in Indian context?

Hi, I am planning to apply for the PhD degree in the Supply Chain Mgt. with specific area of "Cold Storage warehouses" during Pandemics and wars. Where lock downs and shut downs are frequent....

02 March 2021 285 2 View

PACYC PCR not working after several attempts. Would anyone have any suggestions?

So, I have been trying to run a pACYC PCR which will be used later on for a Gibson Assembly. However the PCR is not working. I have already tried gradient PCR and changing extension time; however...

02 March 2021 1,146 2 View

Does anyone know HRM channel in a 2 plex QIAGEN Rotor- Gene Q qPCR can be used as a separate channel?

I am going to have 3 different probes in my qPCR work that I am going to do. But I realized that the machine we have in the lab is a Rotor-Gene Q 2plex HRM Platform, saying it has green, yellow,...

01 March 2021 8,544 1 View

How to address this issue in the analysis of Real-Time PCR results?

To dear Researchers, I was analyzing a series of concentration for estimation of Real-Time PCR efficiency. The concentration was 1:10. I used MS-excel to evaluate Slope. The result of slope was -8...

01 March 2021 8,683 4 View

No title

Does anyone have the experience of using Taq Man probes in the QIAGEN Rotar- Gene qPCR machine?

01 March 2021 5,311 1 View

MiRNA qpcr problem?

Dear All, mirna primer showing some problem in the melting curve? any idea why? As attached is the melting curve. The forward sequence is obtained from miRBase and reverse primer is universal.

28 February 2021 5,008 4 View