11 November 2018 5 8K Report

i am going to buy a real time PCR machine for diagnostic purpose, so please suggest me which is advanced, better and more powerful for diagnostic purpose.

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Is there anybody working in NIPT testing by NGS (Gene studio S5)?

we are planning to buy the gene studio S5 for starting the BIPT testing. we asked and inquired many times for knowing the cost per test for Nipt on above said instrument. i wish to know that what...

03 April 2019 691 0 View

Anybody can suggest a good Giemsa trypsin banding protocol ?

I am using SSC buffer to make a giemsa (20mg per 50 ml SSC buffer). we use aged slide (65 degree for overnight) for treating with Trypsin Solution for 20 sec at 20 degree. We get minor banding...

07 August 2018 4,485 4 View

PGMY11 primer set doesn't give any band with DNA sample which are shown positive with GP5+ and GP6+ primer set of cervical brush sample???

i screened many DNA samples extracted from the cervical brush sample for presence or absence of HPV DNA with primer set GP5+ /6+ and i got five positive sample. then i used that 5 positive samples...

07 August 2017 6,447 2 View

Any one have idea regarding sequence of Primers for Tuberculosis PCR?

There are many primers available in paper published, but most of all have problems with Tm, product size, nonspecific binding and many more. I need a best primer sequences for TB PCR. Is there...

02 March 2017 360 2 View

RAPD analysis of binomial matrix and dendogram preparation?

I have two variety, one is A - in vivo plant leaf and B - in vitro plant leaf. i had extracted DNA from these both samples A & B. Done PCR with 31 RAPD primers and got varies band pattern on...

08 September 2016 8,031 6 View

16s r-DNA PCR product giving three bands on gel, so which one should be considered?

I have Extracted DNA from six different actinomycetes bacterial strains and then do PCR with 27F & 1492R primers. i should get the band with size between 1400 to 1500 bp. but here i got three...

07 August 2016 10,033 6 View

RAPD analysis for the in-vitro and in-vivo plant samples of the same plant species?

I have done RAPD Analysis for one plant with its Invivo tissue and in vitro tissue samples. I have used 31 primer sets, so now i dont getting how much maximum variation can be considered for this...

07 August 2016 899 5 View

What should be the concentration and content of all PCR components for doing RAPD analysis?

i am going to do RAPD analysis of two different plant species with 31 different RAPD primers. I need to know the best suitable concentration and volume of primer, MgCl2, Taq pol, PCR buffer, and...

05 June 2016 994 3 View

Which HPLC method, column type and solvent system will best suite for Alginate analysis extracted from different bacterial strains?

i have extracted Alginate by growing different bacterial strains in broth media. after extracting now have dried alginate as per the extraction protocol.  Now i want to check its purity and want...

05 June 2016 9,727 3 View

How can I remove a high polysaccharide content from DNA extracted with plant leaf?

I have extracted plant DNA from leaf sample. i got good precipitates when precipitated with ethanol and iso propanol. but when i dissolve the dried pellet in TE or molecular grade water, it became...

11 December 2015 4,387 4 View

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