Why the PBS buffer precipitate?

How can i avoid precipitation in pbs? when i solve salts separately then add to a bowel there is no precipitation but when i want to solved them in a bowel after adding 3th salts the salts...

05 June 2018 5,858 4 View

Does Mesenchymal stem cell express Cardiomyocyte cells markers?

Real-Time is done and Mesenchymal stem cells had expression of cTNT, CTNN1 and other Cardiomyocyte cells markers is it possible or cTNT is an specific Cardiomyocyte marker and something wrong is...

05 June 2018 6,515 4 View

Who can give me some information about DRPINs?

09 October 2015 6,419 2 View

Has any one ever detected MCF-7 by flow cytometry with FITC-labeled antibody?

As you know FITC is a dim fluorochrome so I wanna know that FITC is a good fluorochrome for detection of MCF-7?Is there any problem to use of FITC for MCF-7 detection by flow? actually we treated...

31 December 2014 1,428 12 View

Is Isotype control antibodies (mouse IgG) for flow cytometry general?

I'm working on CD34-FITC and used CD34- isotype control antibodies from miltenybiotec company, but the CD34-FITC control is not available in our lab right now but I have CD86-FITC isotype control...

10 November 2014 6,729 8 View

How can I analyze rare cells by fluorescence microscope, not by FACS?

I want to detect some rare cells and I can not do analysis by FACS because of rarity and I have to detect them by fluorescence microscope but I need a protocol for this assay.

10 November 2014 3,439 13 View

Who can send me the photo of normal hematopoitic stem cells which isolated from cord blood?

I wanna know the morphology of them

02 March 2014 9,358 1 View

Is there any site for comparing the levels of expression specific marker between two cells?

I have two cells and i want to know which of them express specific marker more ?

11 December 2013 8,991 3 View

Which company produces specific antigen?

I want to order some antigen and want to know which company is best?

10 November 2013 8,346 4 View

Does anyone know which cancer cell lines (Cancer stem cell, tumor or metastatic) are positive in EpCAM?

I wanna set up a control for my test I want to use some cell lines that the cells are positive in EpCAM.

08 September 2013 7,994 2 View

Why does blocking with my immunogenic peptide cause nuclear fluorescence in ICC?

Hi all, I am validating a polyclonal antibody raised against human ANT4, through WB and ICC. In most of my controls, the antibody shows very little off-target binding, but when I pre-incubate the...

03 March 2021 5,999 3 View

No title

02 March 2021 3,060 3 View

Does Napabucasin soluble in dichloromethane and in ethanol?

I need to prepare a solution of Napabucasin (inhibitor of cancer cell stemness) in dichloromethane. The drug is expensive, so maybe somebody already tried to do it. If it is soluble, what is the...

02 March 2021 9,945 3 View

Help request in CST microwave?

im working now on TPV solar cells and metamaterials and i realy need our help in CST microwave i have a tpv cell simulation and i want to extract particullarly the absorbtion of only the active...

02 March 2021 2,246 2 View

Issues with Permeabilization during Flow Cytometry?

Hello Everyone. Currently I am working to characterize macrophages in the myocardium after ischemia-reperfusion injury in rats. Due to the low total cell number isolated from rat hearts I can...

01 March 2021 3,867 3 View

Which cell line should we use to perform biological dosimetry experiments?

We are preparing some experiments based on irradiating cells under different conditions in order to evaluate the effects in terms of DNA damage, genetic expression, etc. As our project is...

01 March 2021 3,355 3 View

Is it possible for two human neuronal cell lines to have different gene sequence for the same protein?

I am looking at the ATP1A2 (Sodium/Potassium ATPase alpha subunit 2) in two human neuronal cell lines. Expression levels of this protein seems to be almost equal when detected by one antibody....

01 March 2021 3,607 3 View

Is CD44 degraded in lysosome together with hyaluronan after CD44 mediated endocytosis of hyaluronic acid?

Also when RHAMM binds hyaluronic acid, they get internalized, will RHAMM also be degraded? Or both CD44 and RHAMM will be transferred back to the cell membrane? Asking for breast cancer cell line...

01 March 2021 8,169 2 View

Do you need the CMV enhancer before the CMV promotor for a proper function of CMV on the gene that follows?

I'm a student and I have to produce a cell line with a knockin for the NRF2 gene with GFP. I have to put a promotor in front of the GFP because the gene will be too far away from the promotor of...

28 February 2021 7,127 2 View

What could be some reasons why unstimulated monocytes are getting activated?

Im doing PBMC isolation -> CD14+ enrichment using magnetic beads -> stimulation setup. My negative control is just cells in cRPMI but they seem to get activated over and over again.

28 February 2021 7,883 3 View