Inhibition of alpha-glucosidase Enzyme in invitro
I read a few articles and saw that the enzyme concentration they usually use is 0.1U or 0.2U but some articles use 1U. So which one is better and based on what factors do we choose this concentration? Thank you very much.
Adam B Shapiro
The rate of the reaction is directly proportional to the enzyme concentration, as long as initial rate conditions are met. Choose an enzyme concentration that provides a reaction rate that gives you sufficient time to accurately measure the initial rate. If the rate is too fast, you might have difficulty measuring the initial rate. In that case, lower the enzyme concentration to slow down the reaction.
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