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Questions related from Viet Ha

What could be the possible reasons for my plant DNA extraction to appear smeared and degraded on the gel, despite following the CTAB 2% protocol?

Hello everyone, I am currently extracting plant DNA using the 2% CTAB method. The procedure is as follows: Add 0.05g of fresh or dried leaf sample + 1.2 mL of 2% CTAB (containing 1% PVP) + 60 µL...

19 June 2025 4,119 0 View

Is it acceptable to use acetic acid at an oral dose of 1500 mg/kg for analgesia in mice?

I am conducting a pain-induction model using acetic acid 0,9% at a dose of 1500 mg/kg. However, in a book written by a well-known author from my country, it is mentioned that experimental doses...

21 October 2024 705 1 View

What is the concentration of acarbose for Type 2 diabetic mice to drink?

The mice were induced to have type 2 diabetes by feeding high-fat food and injecting low doses of STZ.

08 December 2023 5,715 1 View

Which glucosidase enzyme concentration is better?

Inhibition of alpha-glucosidase Enzyme in invitro I read a few articles and saw that the enzyme concentration they usually use is 0.1U or 0.2U but some articles use 1U. So which one is better and...

02 December 2023 4,931 1 View

Why they dilute at the final step? Do we have to do it and what happen if we don't?

Alpha-Amylase Inhibitory Assay This assay was carried out using a modified procedure of McCue and Shetty [17]. A total of 250 μL of extract (1.25–10 mg/mL) was placed in a tube and 250 μL of...

04 November 2023 4,797 2 View

Are there any standards for raising obese and diabetic mice?

I'm experimenting on Swiss Albino mice and my goal is to make them obese. I don't know if there are any criteria to evaluate whether I have successfully grown fat or not? I read a few articles,...

28 October 2023 5,712 5 View