Has anyone experienced loss of expression by plasmids after floxing their ORFs? It only happened with some promoters types. Secondary structures?

I have floxed an ORF+polyA directly under a neurons specific promoter in this configuration: Neu_promoter-loxP-ORF-polyA-loxP. This seems to impair the transcription since the gen (a reporter) is...

07 July 2016 3,492 0 View