Moving bacteria-like organisms in my HEK293T cell culture?

Hi everyone, I have been culturing HEK293T cells that have been engineered to overexpress the mechanosensitive protein Piezo1. However, I am finding it very difficult to keep them in culture -...

02 February 2021 7,892 4 View

Which fungi species can be employed to hydrolyze insect protein?

I want to perform a solid state fermentation of freeze dried grasshopper and I'd like to know which fungi can be used to hydrolyze the protein from this insect. Thanks in advance!!

27 February 2020 4,638 3 View

How do you interpret a logistic regression odds ratio after transformation?

Hi there Logistic Regression experts, After running a logistic regression and finding that one of my continuous independent variables was not linearly related to the logit of the dependent...

06 February 2020 281 7 View

How to determine the heat transfer areas of a rotary dryer with lifters?

I need to know how to determine the heat transfer areas of a granulated solid in a dryer, the contact area between the solid and the cylinder wall, the area of solid that is in contact with the...

04 September 2018 3,184 3 View

Can anyone suggest an integrity test for ultrafiltration centrifugal devices?

I am working with ultrafiltration centrifugal devices (e.g., nanosep or amicon ultra). I noticed that, depending on the device, there is a certain percent of the devices that fail and allow larger...

05 June 2018 459 0 View

Bumpy melting curves in qrtpcr? I am having some extra picks in my melting curves. I think is primer dimer since it is in NTC?

I am getting some extra bumps in my qrtpcr for actin. I see it in the nyc and in the x10*5 standard. So I think is primer dimer forming when there is not enough template. I tried adding more cDNA...

05 November 2017 5,899 7 View

Is there any research into the consequences of manipulating the normal progression of dementia by utilizing drugs such as Aricept or Namenda?

01 February 2015 8,816 6 View

Does anyone have experience with mixed linear models in panel data?

Does anyone have experience with mixed linear models applied to income data in panel surveys? Annotated examples in stata or spss, or papers about this subject? Thanks a lot!

13 November 2014 6,284 5 View

Are you interested in publishing a paper in a JCR journal related to remote senting? Please check this information

Dear Colleagues, Special Issue on Image Optimization in Remote Sensing Journal of Remote Sensing (ISSN 2072-4292). 2017 Impact Factor: 3.406 (Journal Citation Reports) This special issue...

01 January 1970 7,988 4 View

What is the state of the drug inside solid lipid nanoparticles (SLNs)?

I am a little confuse with the mechanism of solid lipid nanoparticles (SLNs). The main point of encapsulating drugs into SLNs is because some drugs have low solubility in water. Therefore, SLNs...

01 March 2021 3,703 3 View

Isolates of Hymenoscyphus fraxineus?

Dear Dr. Cai, my name is Simone Prospero and I work in the team of Phytopathology at the Swiss Federal Institute for Forest Snow and Landscape Research (WSL;...

01 March 2021 1,133 1 View

How to remove the residual PDMS on the silicon mold?

Incompletely cross-linked pdms are difficult to remove.

01 March 2021 798 3 View

The reason that the culture of white fungi Contamination with green mold(Trichoderma), the culture autoclave at121 for 15 min. What is it?

Is the period to autoclave not enough? The inoculation in a hood and flame and UV and alcohol.

27 February 2021 9,356 3 View

What is the most suitable medium of ascomycetes fungi ??

What is the most suitable medium of ascomycetes fungi ??

26 February 2021 4,413 1 View

Has anyone used the Illumina ITS1 primer sets?

Hi everyone, Illumina provides a list of primers to amplify with high taxonomic coverage the ITS1 region for further fungal sequencing, but I cannot find the exact amount necessary of each...

25 February 2021 6,969 3 View

Have you ever experiencing air vent trouble in fermentation process?

Dear all, My research group is currently working with a 12-litre fermenter. Somehow, if we do the fermentation for more than 20 hours, the process will be more prone to contamination. Our current...

23 February 2021 1,908 2 View

How to overcome convergence issues in concrete arch models?

Hi, I have modelled a three-span arch bridge with soil fill over along with pedestals. The FE modelling is done in LUSAS with HX8M solid elements (i.e. 3D solid modelling). The non-linearity is...

20 February 2021 1,346 2 View

Solid Phase Extraction of drug product containing nanoparticles ?

Hi Everyone, I am trying to develop an SPE method for the vaccine which contains Matrix-M (saponin Quillaja Saponaria ) which is in nano-particle form and size is around 40nm. These nanoparticles...

16 February 2021 1,389 2 View

Can 5-FOA Diffuse Through Agar?

I am preparing to do a gene knockout in the filamentous fungi Coccidioides posadasii targeting pyrG/URA3 so that 5-FOA can be used for selection pressure instead of traditional antibiotic...

16 February 2021 169 4 View