Which fungi species can be employed to hydrolyze insect protein?

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Moving bacteria-like organisms in my HEK293T cell culture?

Hi everyone, I have been culturing HEK293T cells that have been engineered to overexpress the mechanosensitive protein Piezo1. However, I am finding it very difficult to keep them in culture -...

02 February 2021 7,892 4 View

How do I compute alternatives of the Fuzzy Analytic Hierarchy if there are ALOT of them?

I have gotten the average grades of 25 students (They are both Anonymous). These 25 are my alternatives. Criteria from C1............CN are subjects and extracurricular activities. If in one...

20 January 2021 284 3 View

What if People have a VERY different judgment in FAHP?

My research involves player performance in soccer using Fuzzy Analytic Hierarhy Process. I have Tackles, passing, interceptions, shots, etc as my criteria. I have to ask 6 different people and if...

07 July 2020 7,698 1 View

Appearance of crossing point signal even in water during qPCR. Help?

We have been running qPCR to detect Fusarium in wheat grains. Suddenly out of nowhere, we kept getting crossing point signal in the blank (PCR water). We have done the following: ran with only...

01 March 2020 1,408 3 View

How do you interpret a logistic regression odds ratio after transformation?

Hi there Logistic Regression experts, After running a logistic regression and finding that one of my continuous independent variables was not linearly related to the logit of the dependent...

06 February 2020 281 7 View

Does anyone have a good protocol for dissecting the second order mouse pulmonary artery?

Does anyone have a good protocol for dissecting the second order branch of the pulmonary artery from mouse. I am interested in performing some pressure myography studies.

18 December 2019 9,030 1 View

Should non-renewable natural resources be exploited in indigenous territories? yes, no; why?

Consider that the communities disagree, and the government requires economic resources for works and projects (health, housing, roads...).

11 December 2019 4,159 16 View

Should non-renewable natural resources be exploited in indigenous territories? yes, no; why?

Consider that the communities disagree, and the government requires economic resources for works and projects (health, housing, roads...).

01 December 2019 10,634 16 View

How can I ask for permission to reproduce a figure from ThermoFisher.com?

I am currently about to finish my doctoral thesis (hurray!) just to find out that I forgot to ask for permission to some of the figures I used to illustrate it, mostly schemes for the...

28 November 2019 6,104 1 View

Which fungi species yields the highest polysaccharide content in solid state fermentation?

Hello, I''d like to know which fungi can be used to extract the highest content of polysaccharides in a solid state fermentation process. Cheers and thank you in advance!

07 August 2019 5,537 3 View

Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence?

I am on the lookout for the Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence. Does anyone know where I can find it? Thank you in advance

03 March 2021 3,568 1 View

What are the countries most affected by extreme rainfall as a result of climate change?

Increased heat, drought and insect outbreaks, all associated to climate change, have increased wildfires. Decreasing water supplies, declined agricultural yields, health impacts in cities owing to...

01 March 2021 7,168 9 View

What is the state of the drug inside solid lipid nanoparticles (SLNs)?

I am a little confuse with the mechanism of solid lipid nanoparticles (SLNs). The main point of encapsulating drugs into SLNs is because some drugs have low solubility in water. Therefore, SLNs...

01 March 2021 3,703 3 View

Isolates of Hymenoscyphus fraxineus?

Dear Dr. Cai, my name is Simone Prospero and I work in the team of Phytopathology at the Swiss Federal Institute for Forest Snow and Landscape Research (WSL;...

01 March 2021 1,133 1 View

How to accurately quantify chitin?

Hi all, I have been working on the quantification of chitin in insect samples and feed for a while but I can notice that the quantification methods (e.g. exposure to HCl, NaOH, and weighing sample...

01 March 2021 1,843 2 View

How to remove the residual PDMS on the silicon mold?

Incompletely cross-linked pdms are difficult to remove.

01 March 2021 798 3 View

The reason that the culture of white fungi Contamination with green mold(Trichoderma), the culture autoclave at121 for 15 min. What is it?

Is the period to autoclave not enough? The inoculation in a hood and flame and UV and alcohol.

27 February 2021 9,356 3 View

What is the most suitable medium of ascomycetes fungi ??

26 February 2021 4,413 1 View

Has anyone used the Illumina ITS1 primer sets?

Hi everyone, Illumina provides a list of primers to amplify with high taxonomic coverage the ITS1 region for further fungal sequencing, but I cannot find the exact amount necessary of each...

25 February 2021 6,969 3 View

What's the concentration recommend for recombinant protein use in western blotting?

I'm currently working having problem with the concentration for recombinant protein use in western blot, the one been used is Recombinant Human CD19 Fc Chimera Protein-rndsystem which does not...

24 February 2021 8,514 3 View