Moving bacteria-like organisms in my HEK293T cell culture?

Hi everyone, I have been culturing HEK293T cells that have been engineered to overexpress the mechanosensitive protein Piezo1. However, I am finding it very difficult to keep them in culture -...

02 February 2021 7,892 4 View

Which fungi species can be employed to hydrolyze insect protein?

I want to perform a solid state fermentation of freeze dried grasshopper and I'd like to know which fungi can be used to hydrolyze the protein from this insect. Thanks in advance!!

27 February 2020 4,638 3 View

How do you interpret a logistic regression odds ratio after transformation?

Hi there Logistic Regression experts, After running a logistic regression and finding that one of my continuous independent variables was not linearly related to the logit of the dependent...

06 February 2020 281 7 View

Which fungi species yields the highest polysaccharide content in solid state fermentation?

Hello, I''d like to know which fungi can be used to extract the highest content of polysaccharides in a solid state fermentation process. Cheers and thank you in advance!

07 August 2019 5,537 3 View

Where can I find a validated questionnaire that evaluates the professional profile and trajectory of graduates of a residency /specialization program?

I am looking for a validated instrument that allows me to collect information about the exit profile of a residency program and his professional trajectory after the program.

04 June 2019 7,709 3 View

How to determine the heat transfer areas of a rotary dryer with lifters?

I need to know how to determine the heat transfer areas of a granulated solid in a dryer, the contact area between the solid and the cylinder wall, the area of solid that is in contact with the...

04 September 2018 3,184 3 View

Protocol for iNOS detection by flow cytometry?

I will perform an immunoassay to detect the production of iNOS in macrophages by flow cytometry. I need to know what concentration of surfactant I should use to permeate the plasma membrane. Does...

18 August 2018 4,720 3 View

Bumpy melting curves in qrtpcr? I am having some extra picks in my melting curves. I think is primer dimer since it is in NTC?

I am getting some extra bumps in my qrtpcr for actin. I see it in the nyc and in the x10*5 standard. So I think is primer dimer forming when there is not enough template. I tried adding more cDNA...

05 November 2017 5,899 7 View

McIlvaine buffer

A buffer is composed of a weak acid and the corresponding conjugate base, so what is the meaning of a phosphate-citric acid buffer (McIlvaine buffer). In what situations is it used?

02 August 2017 361 2 View

Is there any research into the consequences of manipulating the normal progression of dementia by utilizing drugs such as Aricept or Namenda?

01 February 2015 8,816 6 View

Binary Logistic Generalized Linear Mixed model in SPSS. Is it doable?

I want to analyses the proportion of swimming sperm of three species of fish in two salinities. To analyse the proportion of swimming sperm in a Generalized Linear Model, I would use a Binary...

03 March 2021 2,297 3 View

Is There Any Feasible Method To Test Fluorescent Compounds Other Than Fluorescence Spectrometers ??

Is There Any Feasible Method To Test The Efficiency Of Fluorescent Compounds Other Than UV Spectrometers ? Suggestions Would Be Appreciated !

02 March 2021 5,785 3 View

Does anyone know HRM channel in a 2 plex QIAGEN Rotor- Gene Q qPCR can be used as a separate channel?

I am going to have 3 different probes in my qPCR work that I am going to do. But I realized that the machine we have in the lab is a Rotor-Gene Q 2plex HRM Platform, saying it has green, yellow,...

01 March 2021 8,544 1 View

I have designed reflectarray element and feeding it though Horn Antenna on HFSS. After full wave simulation, I am not getting desired results?

I have designed reflectarray element to generate OAM beams at 5.8GHz and feeding it though Horn Antenna on HFSS. After full wave simulation, i am not getting desired results i.e Radiation pattern...

01 March 2021 9,701 5 View

Samples to study circular dichroism (chirality)?

I want to build a low-cost polarimeter to study circular dichroism (chirality). What type of samples can I use? I am looking for samples that can be found outside the laboratory.

01 March 2021 9,101 3 View

How to accurately quantify chitin?

Hi all, I have been working on the quantification of chitin in insect samples and feed for a while but I can notice that the quantification methods (e.g. exposure to HCl, NaOH, and weighing sample...

01 March 2021 1,843 2 View

Is there a method of determining feed digestibility without the need to re-pellet the feed?

I am aiming to determine the ADC values of a commercial fish feed, but I do not have a steam pelleter or extruder available to me. Ideally, the pellets could be coated with some inert marker....

28 February 2021 7,967 3 View

Good morning research fellows, Has anyone worked with senna alata plant extract on any kind of fish?

Literatures on the piscicidal effect of sennaa alata is scanty. if you have any related litrature either in the bark, leaves or roots used as treatment to any aquatic organism please kindly help...

28 February 2021 9,138 2 View

Can anyone help with Schistosoma identification?

Can anyone identify what kind of Schistosome species this might be? Morphological features of cercariae and adults resemble Trichobilharzia, though I am fairly new to the field of parasitology...

25 February 2021 7,760 6 View

Experimental Design: How deal with pseudreplicates with immunological and clinical-chemical paramaeters?

Hello everybody, I am investigating the influence of a toxinon various clinical-chemical and haematological parameters in fish. I have three treatments (control, low dose, high dose) in my...

24 February 2021 1,243 1 View