Moving bacteria-like organisms in my HEK293T cell culture?

Hi everyone, I have been culturing HEK293T cells that have been engineered to overexpress the mechanosensitive protein Piezo1. However, I am finding it very difficult to keep them in culture -...

02 February 2021 7,892 4 View

How do I compute alternatives of the Fuzzy Analytic Hierarchy if there are ALOT of them?

I have gotten the average grades of 25 students (They are both Anonymous). These 25 are my alternatives. Criteria from C1............CN are subjects and extracurricular activities. If in one...

20 January 2021 284 3 View

What if People have a VERY different judgment in FAHP?

My research involves player performance in soccer using Fuzzy Analytic Hierarhy Process. I have Tackles, passing, interceptions, shots, etc as my criteria. I have to ask 6 different people and if...

07 July 2020 7,698 1 View

Appearance of crossing point signal even in water during qPCR. Help?

We have been running qPCR to detect Fusarium in wheat grains. Suddenly out of nowhere, we kept getting crossing point signal in the blank (PCR water). We have done the following: ran with only...

01 March 2020 1,408 3 View

Which fungi species can be employed to hydrolyze insect protein?

I want to perform a solid state fermentation of freeze dried grasshopper and I'd like to know which fungi can be used to hydrolyze the protein from this insect. Thanks in advance!!

27 February 2020 4,638 3 View

How do you interpret a logistic regression odds ratio after transformation?

Hi there Logistic Regression experts, After running a logistic regression and finding that one of my continuous independent variables was not linearly related to the logit of the dependent...

06 February 2020 281 7 View

Does anyone have a good protocol for dissecting the second order mouse pulmonary artery?

Does anyone have a good protocol for dissecting the second order branch of the pulmonary artery from mouse. I am interested in performing some pressure myography studies.

18 December 2019 9,030 1 View

Should non-renewable natural resources be exploited in indigenous territories? yes, no; why?

Consider that the communities disagree, and the government requires economic resources for works and projects (health, housing, roads...).

11 December 2019 4,159 16 View

Should non-renewable natural resources be exploited in indigenous territories? yes, no; why?

Consider that the communities disagree, and the government requires economic resources for works and projects (health, housing, roads...).

01 December 2019 10,634 16 View

How can I ask for permission to reproduce a figure from ThermoFisher.com?

I am currently about to finish my doctoral thesis (hurray!) just to find out that I forgot to ask for permission to some of the figures I used to illustrate it, mostly schemes for the...

28 November 2019 6,104 1 View

How to quantify bacteria in an unknown sample using qPCR?

I want to quantify the quantity of bifidobacterium in a milk sample. I have extracted DNA from bifidobacteria to be used as my standard, i did a 10-fold dilution and ran my standards with my...

03 April 2019 9,434 4 View

How to quantify bacteria in an unknown sample using qPCR?

I want to quantify the quantity of bifidobacterium in a milk sample. I have extracted DNA from bifidobacteria to be used as my standard, i did a 10-fold dilution and ran my standards with my...

03 April 2019 8,899 3 View

What is an affordable method for producing probiotic pills?

We are isolating probiotic strains from local food samples. What do you suggest to make probiotic pills?

02 March 2019 6,791 6 View

How can to be constant knowledge of what is new in my field so as not to lose my ability to keep abreast of the new application?

How can to be constant knowledge of what is new in my field so as not to lose my ability to keep abreast of the new application?

01 February 2019 7,448 9 View

Does anyone have trouble with molecular characterization of bifidobacteria using universal primers for bacteria?

I have used universal primers for bacteria but it did not work well.

17 January 2019 3,285 4 View

Does anyone have trouble with molecular characterization of bifidobacteria using universal primers for bacteria?

I have used universal primers for bacteria but it did not work well.

31 December 2018 1,999 5 View

How to differentiate Bifidobacterium animalis subsp. lactis BB-12 from Lactobacillus acidophilus la-5 ?

I am currently doing my project on probiotic. I want to grow B. lactis BB-12 using Bifidus Selective Medium (BSM) Agar but my probiotic powder is a mixture of Bifidobacterium lactis BB-12 and...

06 July 2018 3,317 0 View

How i can isolate plasmid from bifidobacterium?

iam woking on bacteriocin isolation from bifidobacterium. please help me how to isolate the plamid from bifidobacterium and how to identify the bacteriocin gene on plasmid?

04 May 2018 9,468 0 View

Are one's fingerprints similar to those of his or her parents?

Fingerprints have a general flow to the ridges that translates into one of three major pattern types: a whorl, loop or arch. It is possible to have just one, two or all three pattern types among...

02 March 2018 603 6 View

QPCR standard curves using bacterial DNA: cloned and not cloned ?

04 May 2017 1,064 2 View