I want to determine the approximate number of UFC/ml mg in a culture.
Thanks in advance!
You can use 600-650nm when the growth in MRS-broth, but this method has big error.
Thank you!!
In MRS broth medium, I could obtained the maximum absorbance at 580nm for Bifidobacterium bifidum and Lactobacillus acidophilus. L. acidophilus have more CFU than B. bifidum at that level.
Thank you very much!
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