We are planning a pot experiment in the greenhouse with forest seedlings and we would like to kill mycorrhizal fungi (basidiomycetes) and to keep ascomycetes alive.
Any ideas on which fungicide we could use? thank you RG!
Hi. I want to kill the basidiomycetes. I want to apply fungicide every now and then to keep the amount of basidiomycetes in the soil as low as possible. Thx!
Hi Jonas! ;-) You will get a bias...but you could grow the soil fungi on media and let them sporulate. As far as I know you need fruiting bodies from the basidiomycetes. So you could make a set of ascomycetes spores from your soil samples and inoculate sterile soil for you pot experiments ??
Hi Arne, the thing is that I will use soil taken from the forest as "inoculum" to be added onto sterile soil. After that I will apply fungicides to modify whatever is in there. One of the treatments aims at killing ascomycetes by applying benomyl, the question is what to apply to supress basidiomycetes...
Jonàs Oliva Zinc Bis, known as Ziram in agriculture, is a fungicide used on a wide variety of plant fungi and diseases. It works on basiodiomycetes.
Dear Jonàs, thank you for posting this interesting technical question on RG. As an inorganic chemist I cannot give you a qualified expert answer. However, I just came across the following potentially useful literature references which might help you in your analysis:
Fungicide selective for basidiomycetes
Article Fungicide Selective for Basidiomycetes
and
Biocontrol Properties of Basidiomycetes: An Overview
Article Biocontrol Properties of Basidiomycetes: An Overview
Unfortunately both articles have not been posted as public full texts on RG. The first article is rather old anyway and most likely outdated. However, you can easily request the full text of the second article from 2017 directly from one of the authors as four of them have RG profiles.
Good luck with your work and best wishes!
Dear, Jonàs Oliva
You can use the fungicide Dithane M45 and Dithane S60 to control some diseases caused by basidiomycetes such as smut diseases.
Also, you can use the fungicides Benlate, Pentachloronitrobenzen (PCNB), Hexachlorobenzen (HCB), Vitavax and Bayletan to control basididiomycetes.
With best regards!
Happy Holidays!
It seems likely that the efficiency of any fungicide may vary between phylogenetically diverse fungi. Mycorrhizal basidiomycetes form also quite a diverse group and you may need a cocktail of compounds to have an overall effect. It seems reasonable to think that there will be trade-offs and undesired knock-outs of species representing specific basidiomycetes and other fungal phyla as well, as for example demonstrated in the following paper:
Fungicide selective for basidiomycetes
L V Edgington, G S Walton, P M Miller
- DOI: 10.1126/science.153.3733.307
Abstract
Concentrations of 2,3-dihydro-5-carboxanilido-6-methyl-1,4-oxathiin lower than 8 parts per million prevented mycelial growth of a number of Basidiomycetes. By contrast, mycelial growth of various other fungi-Phycomycetes, Ascomycetes, and Deuteromycetes-was 50 percent inhibited only by concentrations of 32 ppm or higher. Two exceptions to this pattern of selective fungitoxicity were found:an isolate of Rhizoctonia solani was not as sensitive as other Basidiomycetes, and the deuteromycete Verticillium alboatrum was inhibited by lower concentrations than affected other fungi in this group. Spore germination of two Basidiomycetes, Uromyces phaseoli and Ustilago nuda, was inhibited 95 percent or more at 10 ppm.
Some kind of compromise where you knock out the most prominent basidiomycetes may be achievable through trial of various cocktails. Interesting challenge, Jonas, keep us informed about the progress.
Cheers,
ari
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