Hi!
I'm going to perform a solid state fermentation and I need to inoculate it using Lactobacillus.
Thanks in advance!
For inoculum preparation:
-Inoculate about 35 ml of sterile MRS broth with 300ul of Lactobacillus! (24-48 hr @ 37°C)
-Perform Standard curve for CFU vs. OD600:
A serial dilution for your culture to determine the CFU/mL after vaible count using MRS agar plates.
At the same time serial dilution tubes were saved for optical density measurement at 600 nm (OD600).The fresh MRS broth was used to set blank.
- After data collection, Based on the number of CFU on each agar plate, you can correlate OD600 with CFU to create a standard curve for this specific Lactobacillus.
-From the standard curve you will know which OD are equivalent to your target inoculum(10-7 CFU/mL).
Good luck!
Kindly check https://www.ncbi.nlm.nih.gov/pmc/articles/PMC1797673/
Also check https://www.eppendorf.com/product-media/doc/en/809547/Fermentors-Bioreactors_Application-Note_412_BioFlo-120_320_Microaerobic-Fermentation-Lactobacillus-acidophilus-within-Gut-Microbiome-Physiological-Conditions-BioFlo-Bioprocess-Control-Sta.pdf
The highest concentration of Lactobacillus fermentans was maintained at 26 × 108 CFU / MLO. The Activated Lactobacillus fermentans were put into the container containing culture medium and incubated in anaerobic environment at 35-40 ° C for 10 minutes_ After 20 h, the seed liquid was obtained.The seed liquid was inoculated into the fermentation tank with 36% inoculum by weight percentage, and the fermentation was performed by anaerobic and intermittent stirring.
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