I have extracted RNA from plant immature fruit. I want to synthesize its cDNA by gene specific primers. Please guide me on which (forward or reverse or both) primers are used in such cases.
Strand-specific primers are to be used with viral qPCR...(and some bacterial qPCR)... but in your case, reverse primer is correct as Sanchita says. The reverse primer will be thus used in reverse-transcribing RNA to cDNA that is then anti-sense in orientation with respect to the coding strand of the gDNA sequence. To which, then, the forward primer can then interact... etc. Long story made short. Orientation of priming is becoming more and more of an issue as we learn more and more these days. So this is a very good question (whereas it used to be thought of as 'an obviously bad question')... now, it is good to make this specific distinction in most situations.
Dear Dr. Rangbar
reverse primer for positive stranded virus or mRNA and forward primer for negative senseRNA viruses are recommend for cDNA synthesis
good luck
Primer use for cDNA synthesis base on polarity of RNA, if RNA negative sense must use forward primer but if RNA positive sense must use reverse primer.
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