Would RNA extraction from plants be successful if i do not use Liquid nitrogen?

Usman Ali @Usman_Ali15

03 March 2014 1 4K Report

Thanks in advance

Magdalena Stolarek

I don't have to use the liquid nitrogen, you can just grind material in the trizol all attached buffer to the kit.

Badges
Science topic

More Usman Ali's questions See All

What are the primers to get whole length of the sequence?

Dear all Please if someone can guide me.. I have cloned a gene of 1500 bp pairs in FAST-Plasmid. I used reverse primers to sequence the gene. The company sent me 900bp length.. I want to sequence...

11 December 2015 8,850 1 View

Anyone familiar with restriction double digestion of p35S-FAST Plasmid?

Hello everyone I want to double digest p35S-FAST Plasmid with Kpn1 and BamH1. The double digest calculator-Thermo scientific recommends the following condition. BamHI buffer2-fold excess of...

10 November 2015 7,218 2 View

Restriction double digestion of p35S-FAST Plasmid..?

Hello every one I want to double digest p35S-FAST Plasmid with Kpn1 and BamH1 1X tango buffer.. The "Double Digest Calculator-Thermo Scientific" recommends the following condition. 1X Tango...

10 November 2015 4,612 0 View

Which (forward or reverse, or both) primers should I use for cDNA synthesis from gene specific primers?

I have extracted RNA from plant immature fruit. I want to synthesize its cDNA by gene specific primers. Please guide me on which (forward or reverse or both) primers are used in such cases.

03 April 2014 9,431 6 View

Can you help me achieve adequate amplification of required band?

I am using 0.2mM dNTPs,40pm primers,0.6microlitre Taq,2 microlitre MgCl2(2.5mM) in a total 20 microlitre reaction volume for PCR. I have kept the temperature the same so far. I need a band size of...

02 March 2014 1,531 13 View

Would the process of extracting RNA from plants without liquid nitrogen be successful?

02 March 2014 4,493 9 View

Can anyone help with RNA storage?

I stored plant RNA samples at -20 degree centigrade for 5 days and then I shifted to -80 degrees. Can I use this RNA for cDNA synthesis?

01 February 2014 6,069 7 View

How should my cDNA band look like on gel?

I want to synthesize cDNA from plant RNA. Please tell me that what should the cDNA band look like on gel, so that I can guess that cDNA has successfully synthesized. Please show me some gel...

01 February 2014 1,002 1 View

Can anyone help with optimizing PCR conditions for my gene of interest?

I made different dilutions of control cDNA and in each case after running its PCR got the band of 496bp .The Gel pic is as below. But when I ran PCR for my gene of interest with same dilutions of...

01 February 2014 715 3 View

Can anyone help with cDNA agarose gel?

I have synthesised cDNA from plant RNA .The gel picture is given below. Please if anyone could tell me that whether the cDNA has synthesised or not? If synthesised, can I use this for Reverse...

01 February 2014 3,460 13 View

Is there a problem with my RNA pellet?

Hello, I am currently having problems with RNA extraction. I am using mouse liver (C57BL6J), and I have extracted RNA from mouse liver before. Before this experiment, my final RNA pellets were...

11 August 2024 7,082 3 View

Strugglling with m6A dot blot any suugesstion ?

I have been doing the m6A dot blot for a while with no improvement, I am extracting the RNA, and I can see the dots although the three biological replicas give a different reading on the memberan...

10 August 2024 8,539 5 View

Could it be a cell culture contamination?

Hello everyone! I observed in my culture (htert-RPE1 cells) an orange- red particle at the bottom of the dish. It is visible to the naked eye as a very very small red dot. Could it be a...

09 August 2024 2,824 3 View

RNA Extraction Using Hot Borate Method No Longer Working?

I've been performing RNA extraction on cotton petiole tissue for a few months now using the method described in the following paper, a derivative of the typical hot borate method...

08 August 2024 9,882 2 View

Does Anyone have expertise in in vitro transcription and RNA pull down assay?

I am currently working on LncRNA; to know the lncRNA-protein interactions I want to do RNA pull down assay, so I need to design primers with T7 promoter. I need assistance in this regard.

07 August 2024 6,622 1 View

Microtome tissue sectioning - How to prevent chatter of paraffin tissue specimen sections?

Hello everyone, I have recently started using the microtome device for sectioning of paraffin-embedded mice lung. While I had some success in sectioning and observing proper ribbons, some of my...

06 August 2024 666 4 View

E.coli contamination in human RNA seq data ?

Recently, we observed that 99% of the sequences in our RNA-seq data corresponded to the E. coli genome. Despite multiple DNAse treatments after RNA extraction and ribosomal depletion, we were...

06 August 2024 807 3 View

How do soil microflora interact with plant roots and influence plant nutrition, health, and productivity?

06 August 2024 9,618 3 View

RNA later for the preservation of RNA in fecal samples at room temperature for one day (37°C)?

I am planning to collect human fecal samples for metatranscriptomic analysis using MGI. These samples are from indigenous people living in a region with high temperatures. I will have access to a...

06 August 2024 1,367 3 View

Weak DAPI staining after immunohistochemistry - how to improve?

After immunohistochemistry of previously fixed in PFA and EtOH and then frozen 20 μm sections of zebrafish brain, DAPI staining is very weak (right) compared to the same sections stained without...

05 August 2024 9,637 2 View