Usman Ali

11 Questions 8 Answers 0 Followers

Questions related from Usman Ali

What are the primers to get whole length of the sequence?

Dear all Please if someone can guide me.. I have cloned a gene of 1500 bp pairs in FAST-Plasmid. I used reverse primers to sequence the gene. The company sent me 900bp length.. I want to sequence...

12 December 2015 8,833 1 View

Anyone familiar with restriction double digestion of p35S-FAST Plasmid?

Hello everyone I want to double digest p35S-FAST Plasmid with Kpn1 and BamH1. The double digest calculator-Thermo scientific recommends the following condition. BamHI buffer2-fold excess of...

11 November 2015 7,202 2 View

Restriction double digestion of p35S-FAST Plasmid..?

Hello every one I want to double digest p35S-FAST Plasmid with Kpn1 and BamH1 1X tango buffer.. The "Double Digest Calculator-Thermo Scientific" recommends the following condition. 1X Tango...

11 November 2015 4,548 0 View

Which (forward or reverse, or both) primers should I use for cDNA synthesis from gene specific primers?

I have extracted RNA from plant immature fruit. I want to synthesize its cDNA by gene specific primers. Please guide me on which (forward or reverse or both) primers are used in such cases.

04 April 2014 9,418 6 View

Can you help me achieve adequate amplification of required band?

I am using 0.2mM dNTPs,40pm primers,0.6microlitre Taq,2 microlitre MgCl2(2.5mM) in a total 20 microlitre reaction volume for PCR. I have kept the temperature the same so far. I need a band size of...

03 March 2014 1,512 13 View

Would the process of extracting RNA from plants without liquid nitrogen be successful?

03 March 2014 4,477 9 View

Would RNA extraction from plants be successful if i do not use Liquid nitrogen?

Thanks in advance

03 March 2014 4,044 1 View

Can anyone help with RNA storage?

I stored plant RNA samples at -20 degree centigrade for 5 days and then I shifted to -80 degrees. Can I use this RNA for cDNA synthesis?

02 February 2014 6,053 7 View

How should my cDNA band look like on gel?

I want to synthesize cDNA from plant RNA. Please tell me that what should the cDNA band look like on gel, so that I can guess that cDNA has successfully synthesized. Please show me some gel...

02 February 2014 980 1 View

Can anyone help with optimizing PCR conditions for my gene of interest?

I made different dilutions of control cDNA and in each case after running its PCR got the band of 496bp .The Gel pic is as below. But when I ran PCR for my gene of interest with same dilutions of...

02 February 2014 699 3 View

Can anyone help with cDNA agarose gel?

I have synthesised cDNA from plant RNA .The gel picture is given below. Please if anyone could tell me that whether the cDNA has synthesised or not? If synthesised, can I use this for Reverse...

02 February 2014 3,441 13 View