Sanchita Dey

4 Questions 3 Answers 0 Followers

Questions related from Sanchita Dey

Which vector works best for large scale purification of protein using saccharomyces cerevisiae as an expression system?

I have been trying to express an eukaryotic protein in E.coli, but in vain. Their have been no induction using IPTG and the vector was of pet series. So, I have planned to clone it in a...

11 November 2017 2,127 0 View

My protein seems to oligomerize into higher order soluble aggregates as seen after FPLC. How can i prevent it so as to set crystalization trials?

My protein after gel filtration has a tendency to oligomerize into numerous soluble ologomers. What will aid in shifting this equilibria of protein towards any single homomeric state, so that I...

10 October 2017 7,321 6 View

Could you please suggest a protocol which is best suited for electroporation of filamentous fungus Trichophyton rubrum?

Minute details will be very much of my interest. Also, conidial germination time along with details of growth media, amount of plasmid DNA and other electroporation parameters will be of much...

07 July 2014 6,136 2 View

Which is the best tag to purify serine proteases and what cells are best to use for optimum induction?

I am finding it difficult in a large scale purification to use this tag. Also, I am not getting optimum expression of my protein by inducing at both 37 and 18 degrees as well. Rosetta DE3, C43 and...

12 December 2013 8,405 2 View