Hi, I recently got a specific human T-cell line and in order to study antigen presentation transformed autologous B-cell with Epstein-Bar virus.
Now, I would like to irradiate the EBV-B-cell to prevent their proliferation retaining their antigen processing and presenting characteristic.
I can only have access to an X-ray machine suitable for mice, does anybody have experience in this type of assay ?? How many gy should I irradiate with ??
Thanks a lot
Cheers
Dario Leone
I tested this last year over doses between 30, 50 and 70 gy on EBV B cell lines. I ended up finding that 50gy was optimal in terms of preventing proliferation, but retaining antigen processing and presentation characteristics. 70gy resulted in high cell death, and 30gy resulted in some proliferation.
Best,
Jordon Dunham
to prevent proliferation of non transformed B cells,30 Gy is enough but for transformed B cell ,need to increase to 40 Gy.
good luck,
Mohammad,
Hi, does this dose (50 gy) also inactivate the EBV virus completely ?
I want to use supernatant of EBV infected B cells in a HEK293 transfection experiment and thus need to inactivate any virus present in the supernatant in order to avoid the risk of spreading infection in the host cell .
Many thanks,
Jakir
I'm not sure about viral inactivation or shedding. This would depend on the line you are working with.
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