Dario Armando Leone

4 Questions 6 Answers 0 Followers

Questions related from Dario Armando Leone

How can I analyse cytokine present within tumor microenvironment ?

I would like to determine the cytokine present within the tumor microenvironment and I was wondering if anybody was able to detect them in the dissociation media used to generate the single cell...

08 July 2020 6,629 0 View

Is there a company that sale HLA specific buffy coat?

I got a CD4 cmv restricted cell line isolated and expanded from a patients that is HLA Class II: DR1*01 DR4 but in order to perform the proliferation assay I would need APCs derived from a...

19 June 2015 8,099 2 View

Why do I lose 50%-60% of T-cells after staining with CFSE or CPD?

I am setting up an antigen proliferation assay using Monocytes Derived Dendritic pulsed with KLH in co-culture with lymphocytes isolated by elutriation technique. I perform a CD4 negative...

30 April 2013 7,689 19 View

Common plasma membrane marker for segmentation

Hi there, as spatial biology became very popular lately I am wondering whether it exist a common plasma membrane marker, like laminin for the nuclear membrane able to be used on histological...

01 January 1970 2,987 0 View