Dario Armando Leone

6 Questions 8 Answers 0 Followers

Questions related from Dario Armando Leone

How can I analyse cytokine present within tumor microenvironment ?

I would like to determine the cytokine present within the tumor microenvironment and I was wondering if anybody was able to detect them in the dissociation media used to generate the single cell...

08 July 2020 6,686 0 View

Why peptide and not native antigen are mostly used for antibody production and in vitro T-cell stimulation ?

A part the technical and practical reason, linear peptide are much more easy to produce and isolate, is there a biological reason that support the idea that peptide are more efficient than native...

22 June 2020 6,823 4 View

Which dose of X-ray should I use for irradiating EBV-transformed B-cell used as antigen presenting cells co-cultured with T-cell ?

Hi, I recently got a specific human T-cell line and in order to study antigen presentation transformed autologous B-cell with Epstein-Bar virus. Now, I would like to irradiate the EBV-B-cell to...

27 October 2015 6,539 4 View

Is there a company that sale HLA specific buffy coat?

I got a CD4 cmv restricted cell line isolated and expanded from a patients that is HLA Class II: DR1*01 DR4 but in order to perform the proliferation assay I would need APCs derived from a...

19 June 2015 8,152 2 View

Why do I lose 50%-60% of T-cells after staining with CFSE or CPD?

I am setting up an antigen proliferation assay using Monocytes Derived Dendritic pulsed with KLH in co-culture with lymphocytes isolated by elutriation technique. I perform a CD4 negative...

30 April 2013 7,740 19 View

Common plasma membrane marker for segmentation

Hi there, as spatial biology became very popular lately I am wondering whether it exist a common plasma membrane marker, like laminin for the nuclear membrane able to be used on histological...

01 January 1970 3,046 0 View