Enzymatic activity
13 December 2020 1,559 3 View
Dear all, I have an issue figuring out good plunging conditions for large lipid structures: I have carbon coated C-Flat 2/2 400mesh copper grids with large (elongated) lipid structures on it....
13 December 2020 6,696 2 View
Hi, I generally made LV in HEK293T cell lines by triple transfection. I am wondering whether the newly generated LV will transduce the packaging HEK293T. In this way I could exploit the...
09 September 2020 6,333 3 View
I usually use Beamer, in which equations are easy to write (and I already have them in Latex) but it's too static and rigid. In Powerpoint it is impossible to write the equations, it takes a lot...
15 July 2020 8,260 3 View
I'm calculating the max available Jsc as a function of bandag Eg. For that I'm using the following integral: Jsc=q*int(f(Eph)*EQE.dEph) from Eg to \inf With: q=1C/eV f(Eph) Spectral Irradiance...
07 July 2020 5,873 4 View
Dear all, I'm trying to calibrate an assay for FIHC of BMP4 in whole mouse embryos, Recommendation for FIHC assay that would help me to detect the secreted protein would be highly appreciated!
16 April 2020 9,547 3 View
Analyzing the topology of the LM741 , the current mirror (Q8-Q9) that bias the first stage of the op. amp. is not connected as usual (see the red circle on the picture). Curiosly, this way to...
24 January 2020 8,419 2 View
I am performing in vitro anti-inflammatory assay: inhibiton of albumin denaturation by organic extract and purified fractions from natural material. Since my samples are colored (red, orange,...
14 September 2019 2,528 1 View
Hi to all, I have an approaching deadline and to perform my analysis (design guide RNAs) I need to insert a lot of mutations in my sequenze (txt file). Mutations are inside a excel in file...
05 February 2019 2,494 1 View
I've read that, for students in the spectrum, it may help to design a class environment that avoids overstimulating their cognitive system with unnecessary distractions, one of them begin colorful...
28 January 2019 2,557 3 View
24 February 2021 1,365 3 View
I do flavonoid test for biscuit incorporated with muntingia fruit powder. Sample extracted with metanol (1:10 ,w/v), macerated 24h, sentrifuged and filtered. Supernatan used for analysis. I have...
08 February 2021 6,779 4 View
Environmental Impact Assessment (EIA) does not live up to the expectation of environmental lawyers and environmental engineers. In your own opinion, how can the EIA process therefore be made more...
03 February 2021 1,329 6 View
01 February 2021 4,616 3 View
01 February 2021 1,825 1 View
I want to examine a kind of fruit's extract's antimicrobial effects. At first I want to put it under the UV radiation to prevent the mold or any kind of fungi's and bacteria's growth. The...
24 January 2021 8,350 20 View
I'm measuring fluorescence intensity of the product in an enzyme reaction in 37 celsius degree. I set a kinetic measurement in 1 hour. The fluorescence intensity of the BLANK ( contains only...
03 January 2021 5,554 5 View
It is known that scientific knowledge is replicated each time at more unusual speeds, however, humanity expects more from our scientific work, that is, solutions to problems that have accumulated...
02 January 2021 8,300 16 View
The chromogenic beta-lactamase substrate CENTA is at least five times cheaper than the other famous substrate nitrocefin and can be used with most enzymes, still, nitrocefin is preferred. Any...
17 December 2020 3,735 2 View
Hi every one, I am extracting enzymatic extract from plant (50 mM Sodium phosphate buffer (pH 7.8), 0.1 mM EDTA, 0.1 % (v/v) Triton; 1 mM PMSF). I mesure the protein content and enzymatic...
10 December 2020 7,759 2 View
