Hi everyone,
I need to buy a column for my HPLC-MS; I analyze samples that contain a PEG-small molecule conjugate in order to quantify the small molecule that is released from the PEG backbone. The PEG is 6kDa and the small molecule is 400 Da.
The supplier of the instrument suggested one of their column, a C18 50mm.×2.1mm, 2.7μm, pore size 90A.
After not so long, I started having back pressure problems and now it's impossible to use. I was wondering if you think that PEG could accumulate/interact with the stationary phase or something else. Do you think the pore size or the thin internal diameter could have had a role in this?
Should I purchase a column that has specific characteristics?
thank you!
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