Hei,

I induced differentiation in various neuroblastoma cell lines:

  • SKNBE(2)
  • CHLA-20
  • SHSY5Y
  • Kelly
  • In addition, I will analyze differentiation in SKNDZ

    In order to show that differentiation is induced, I will analyze for the expression of differentiation markers by westernBlot.

    Which differentiation markers should I analyze? Which may be the best for the cell lines I am using?

    Right now, we have the following antibodies in the lab:

  • NSE
  • β-Tubulin (Neuronal Class III -βTubulin)
  • MsX Tyrosine Hydroxylase
  • Gap43 (but the antibody is so unspecific that we will buy a new one)
  • Bcl2
  • Neurofilament Heavy Tail Domain
  • Neuro D (A-10)
  • Do you use other markers to show differentiation by Western Blot? Which are working best?

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