Sarah A. Roth

12 Questions 15 Answers 0 Followers

Questions related from Sarah A. Roth

How to run qualimap (command-line tool)?

I tried to run qualimap: http://qualimap.bioinfo.cipf.es/. When using the command ./qualimap, I am always getting the following error message: ./qualimap Java memory size is set to...

24 September 2018 9,541 3 View

Which DNA isolation Kit for NGS (MiSeq, WGS of bacteria)?

Hello, for routine work, we are using the QIAamp DNA Mini Kit to isolate the DNA from bacteria. This kit works fine when the intention is to use the DNA for routine PCR. However, the yield is...

24 January 2018 8,376 4 View

Why using Insulin-Transferrin-Selenium (ITS) for cell culture?

I was wondering why some cells in culture should be grown in the presence of Insulin-Transferrin-Selenium (ITS). I understand why insulin may be important for the growth of some cells but why may...

18 January 2017 6,577 3 View

What is the 1p status of the neuroblastoma cell lines CHLA-15 and CHLA-20?

Hei, I cannot find any paper describing the 1p(36) status of CHLA-20 and CHLA-15. Does anyone know a paper showing if these cell line have 1p36 LOH/Deletions?

20 December 2016 7,265 3 View

Bands with shadows- what happened to my western Blot?

Hei, Today I got a very strange western Blot. Normally, the antibody is working perfectly but today, the bands are looking very strange- there are shadows around the bands (see attachment). What...

31 May 2016 8,186 7 View

Is there someone who has a 3`UTR-luciferase plasmid (CyclinD and Mcl1)?

We are looking for a luciferase reporter plasmid containing the 3`UTR of CyclinD1 or Mcl-1 (preferable the whole 3`-UTR). It is so frustrating- we have written circa 20 emails to research groups...

02 March 2016 990 0 View

May binding of miRNAs to the 3'UTR stabilize mRNA while inhibiting translation?

Hei, I used RT-PCR to analyze whether predicted or verified targets of a miRNA are actually targets of this miRNA in my cell lines. For some of these genes I found either a slight but clear...

05 February 2016 999 3 View

How may I analyze drug sensitivity in differentiated neuroblastoma cell lines?

I have the following issue: I am currently working on the role of a miRNA (I will call this miRNA miR-X) on drug sensitivity in neuroblastoma (see figure pdf). I found that this miRNA...

08 October 2015 5,852 3 View

Can anyone help me with expression data analysis using the software ingenuity (IPA)?

I want to analyze microRNA expression data using ingenuity. I have a lot of questions concerning this analysis. Unfortunately, it is very difficult to get support directly from ingenuity. I have...

23 April 2014 9,348 1 View

Horseshoe shaped nuclei after treatment with a DNA-damaging agent?

Hey, I overexpressed a gene of interest and treated cells with a DNA-damaging agent followed by staining with Hoechst at different time points. Many of my cells overexpressing the gene of...

18 March 2014 5,812 4 View

Has anyone heard about pseudomonolayers?

Currently I am working with the neuroblastoma cell line SK-N-BE(2). Today I stained the nuclei using Hoechst and analyzed the cells under the microscope. I was surprised when I found that control...

14 March 2014 7,388 3 View

SK-N-BE(2) and analysis of apoptosis using Annexin V-FITC?

I am using Annexin V-FITC to analyze apoptosis by flow cytometry. Currently, I am working with the two neuroblastoma cell lines SK-N-BE(2) and SK-N-AS. The assay works perfect using the...

23 January 2014 3,844 3 View