22 Questions 29 Answers 0 Followers
Questions related from Sarah A. Roth
I tried to run qualimap: http://qualimap.bioinfo.cipf.es/. When using the command ./qualimap, I am always getting the following error message: ./qualimap Java memory size is set to...
24 September 2018 9,639 3 View
Hello, for routine work, we are using the QIAamp DNA Mini Kit to isolate the DNA from bacteria. This kit works fine when the intention is to use the DNA for routine PCR. However, the yield is...
24 January 2018 8,471 4 View
I was wondering why some cells in culture should be grown in the presence of Insulin-Transferrin-Selenium (ITS). I understand why insulin may be important for the growth of some cells but why may...
18 January 2017 6,626 3 View
Hei, I cannot find any paper describing the 1p(36) status of CHLA-20 and CHLA-15. Does anyone know a paper showing if these cell line have 1p36 LOH/Deletions?
20 December 2016 7,325 3 View
Hei, Today I got a very strange western Blot. Normally, the antibody is working perfectly but today, the bands are looking very strange- there are shadows around the bands (see attachment). What...
31 May 2016 8,229 7 View
We are looking for a luciferase reporter plasmid containing the 3`UTR of CyclinD1 or Mcl-1 (preferable the whole 3`-UTR). It is so frustrating- we have written circa 20 emails to research groups...
02 March 2016 1,022 0 View
Hei, I used RT-PCR to analyze whether predicted or verified targets of a miRNA are actually targets of this miRNA in my cell lines. For some of these genes I found either a slight but clear...
05 February 2016 1,037 3 View
I want to know how Max may be regulated at the transcriptional level (by transcription factors). What are factors that inhibit or promote Max expression on the trascriptional level? Miao et al....
08 January 2016 8,679 5 View
Can someone explain me what the Delta Cell index is? I know how it is calculated but why using the Delta Cell index instead of the normalized cell index?
17 December 2015 7,091 8 View
Hei, I analyzed caspase3/7 activation (using the Kit from Promega) 24, 48, 72 and 96 hours after treatment (se figure pdf). Caspase activation has increased 24 hours after treatment, is highest...
27 November 2015 8,113 2 View
I want to analyze apoptosis by flow cytometry using FITC-Annexin V and Propidium Iodide (PI). In order to be able to set gates (indicating AnnexinV or PI-positive or negative cells) and to set the...
18 November 2015 9,205 4 View
Hei, I have the following issue: I found a "something" (I do not want to tell too much ;-) ) that induces differentiation in my neuroblastoma cell lines. Microscopy indicates that it induces...
03 November 2015 6,954 7 View
Hei, I induced differentiation in various neuroblastoma cell lines: SKNBE(2) CHLA-20 SHSY5Y Kelly In addition, I will analyze differentiation in SKNDZ In order to show that differentiation is...
20 October 2015 3,527 4 View
Hei, I just started to work on neuroblastoma differentiation... I am looking for reviews explaining what is happening in the cell when they are differentiating. I found a paper describing a tumor...
13 October 2015 9,136 3 View
I have the following issue: I am currently working on the role of a miRNA (I will call this miRNA miR-X) on drug sensitivity in neuroblastoma (see figure pdf). I found that this miRNA...
08 October 2015 5,892 3 View
I want to analyze microRNA expression data using ingenuity. I have a lot of questions concerning this analysis. Unfortunately, it is very difficult to get support directly from ingenuity. I have...
23 April 2014 9,390 1 View
Hey, I overexpressed a gene of interest and treated cells with a DNA-damaging agent followed by staining with Hoechst at different time points. Many of my cells overexpressing the gene of...
18 March 2014 5,866 4 View
Currently I am working with the neuroblastoma cell line SK-N-BE(2). Today I stained the nuclei using Hoechst and analyzed the cells under the microscope. I was surprised when I found that control...
14 March 2014 7,425 3 View
I am currently investigating doxorubicin-mediated cell death. Therefore, I also want to analyze whether cells might die of apoptosis and whether apoptosis might be caspase-dependent or...
24 February 2014 568 35 View
Thanks in advance.
19 February 2014 1,524 3 View
Some cells (especially those with non- functional p53) are not able to arrest in G1 in response to treatment with for example DNA-damaging agents. Those cells often rely on the G2 checkpoint for...
30 January 2014 5,554 7 View
I am using Annexin V-FITC to analyze apoptosis by flow cytometry. Currently, I am working with the two neuroblastoma cell lines SK-N-BE(2) and SK-N-AS. The assay works perfect using the...
23 January 2014 3,883 3 View
