Which software do you use for biological FIB-SEM data segmentation?

Dear colleagues, A good FIB-SEM data stack can contain a few thousands pictures. It is very tricky and annoying process to segment them all manually. Which software do you use (real practical...

01 February 2019 2,914 8 View

Which chemical reaction makes HM20 resin pink?

The datasheet says: "After polymerization, the block sometimes turns to a light pink or pink/red color. This seems to be quite normal and should not be a cause for concern. " Indeed, after...

31 December 2018 5,513 6 View

Do you have experience with high pressure freezing (Leica EM PACT2 or ICE) of very soft tissues (testis, etc)?

Dear colleagues, Did you use HPF for some super-soft tissue like testis? Operation with "sampled" specimens like pieces of tissue, small worms, etc. is pretty clear. But when we have some...

09 October 2018 7,461 15 View

Which time between dissection and high pressure freezing is acceptable?

Dear colleagues, Obviously, this time should be as short as possible, but sometimes we need to dissect animals in one building and transfer the samples to another. Which time between dissection...

09 October 2018 6,230 15 View

How do you conduct freeze substitution (Leica AFS) for 6 mm sapphire discs?

Dear colleagues, We have prepared a few 6 mm sapphire discs with cells monolayers by HPF, they are in liquid nitrogen now. These disks are too big for conventional Leica freeze substitution...

05 June 2018 7,979 8 View

Which design of blocks do you prefer for FIB-SEM tomography?

Dear colleagues, Preparation of blocks for FIB-SEM tomography is a little tricky process. Which design do you prefer and which technique and tricks use in the preparation? I make the blocks...

05 June 2018 707 1 View

Does Zika virus have a serious pandemic potential?

31 December 2015 9,104 19 View

How to compare two groups with only two measurements?

Hiiiii everyone! I have an enquiry on statistical analysis. I was looking for many forum and it's still cannot solve my problem. I want to compare means of two groups of data but only with two...

03 March 2021 8,796 3 View

Can we classify HV and LV lines based on line length?

I have dataset which shows the length of power lines. I need to classify the lines based on the line length. Is there a rule to classify the High voltage (HV) and low voltage (LV) lines based on...

03 March 2021 4,116 4 View

Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence?

I am on the lookout for the Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence. Does anyone know where I can find it? Thank you in advance

03 March 2021 3,568 1 View

What kind of fluid could I use for a pitfall trap that also does not invalidate molecular testing?

Hi, I want to start testing pitfall trap to obtain ants samples, but I need to conduct molecular analysis on those insects. So, what kind of fluid can I use? Ethanol expires too early and I need...

03 March 2021 5,978 5 View

What's the best way to measure growth rates in House sparrow chicks from day 2 to day 10?

What's the best way to measure growth rates in House sparrow chicks from day 2 to day 10? Since, the growth curve from day 2 to 10 won't be like the "Logistic curve" it might not follow logistic...

03 March 2021 1,401 3 View

Does any one know how i can prepare liver tissue with transmission electron microscope with good fixation?

i have problem in preparation of liver tissue with bad cell organelles and how i can adjust osmolarity

03 March 2021 8,890 1 View

Do we need to write protocol for a second study of a systematic review that has been registered and published?

I have conducted and published a systematic review and meta-analysis research with the topic related to public health and health pomotion (protocol was registed in PROSPERO). Now we want to...

03 March 2021 8,920 3 View

How to increase a model accuracy ?

dear community, my model is based feature extraction from non stationary signals using discrete Wavelet Transform and then using statistical features then machine learning classifiers in order to...

03 March 2021 6,994 5 View

What is the production process of CaFe ferroalloys?

I looking for ferro calcium production process

03 March 2021 1,755 4 View

I need to image mesoporous silica nanoparticles that are in ~100 nm size and do high resolution TEM to look at mesopores. what grids should I use?

Need to image mesoporous silica nanoparticles using the TEM. Also, need high resolution TEM images to see the mesoporous structure. Kindly suggest what kind of grids to use. Thanks, Shatadru

02 March 2021 1,787 2 View