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Questions related from Denis Korneev
Dear colleagues, A good FIB-SEM data stack can contain a few thousands pictures. It is very tricky and annoying process to segment them all manually. Which software do you use (real practical...
02 February 2019 3,021 8 View
The datasheet says: "After polymerization, the block sometimes turns to a light pink or pink/red color. This seems to be quite normal and should not be a cause for concern. " Indeed, after...
01 January 2019 5,653 6 View
Dear colleagues, Obviously, this time should be as short as possible, but sometimes we need to dissect animals in one building and transfer the samples to another. Which time between dissection...
10 October 2018 6,329 15 View
Dear colleagues, Did you use HPF for some super-soft tissue like testis? Operation with "sampled" specimens like pieces of tissue, small worms, etc. is pretty clear. But when we have some...
10 October 2018 7,575 15 View
Dear colleagues, Preparation of blocks for FIB-SEM tomography is a little tricky process. Which design do you prefer and which technique and tricks use in the preparation? I make the blocks...
06 June 2018 810 1 View
Dear colleagues, We have prepared a few 6 mm sapphire discs with cells monolayers by HPF, they are in liquid nitrogen now. These disks are too big for conventional Leica freeze substitution...
06 June 2018 8,093 8 View
Dear colleagues, I defended my Ph.D. thesis in October 2016 and now I am looking for a postdoctoral position in microscopy (AFM, TEM, SEM) and biophysics of microorganisms (especially, viruses, I...
05 May 2017 1,333 7 View
Last week we have read a lot of "dramatic" news about this virus. For example: http://www.bbc.com/news/world-us-canada-35425731 Is this problem really serious or it is just dramatization by some...
01 January 2016 9,203 19 View
