I want to culture methanogens from the fecal sludge sample. Which culture media should I use?
I am doing driving style analysis research with OBD data. Could anyone provide me links to get the dataset?I am not able to get it anywhere..
20 January 2021 9,707 2 View
Is a person who smokes at a higher risk of getting affected by corona virus than that a non-smoker?
14 January 2021 4,246 30 View
I have a panel data of monthly milk yield as a dependent variable and extreme weather ( in terms of percentile-based days) and other independent variables on the other side of the equation. The...
04 October 2020 8,960 1 View
How can I add atomic parameters for the ions Europium (Eu3+) and Americium (Am3+)
04 October 2020 1,718 6 View
I am looking fora command that will modify 3 chains available in the original pdb into a single chain and then renumber all of the residues. I have tried using alter command but when I export the...
26 June 2020 8,913 1 View
I understand that when a material say, Fe is doped in say, ZnS nanoparticles, then there are no extra phases formed and there is only a shift in XRD patterns observed. But when nanocomposite is...
06 June 2020 2,255 6 View
what are the quantities that confirms formation of dimer if we simulate two monomer with specific force field
13 February 2020 283 13 View
https://www.surveymonkey.com/r/CB72MG6
08 February 2020 4,048 1 View
I want to quantify a protein of ~ 7 kDa ( not a recombinant or amplified) from the crude protein extract. The sequence of the protein is known and has only one UV absorbing aminoacid.
04 February 2020 7,574 4 View
I understand from the literature that child reports are more accurate than parent reports for school children, but wanted to specifically look at a brief ffq of parents for the same .
04 April 2019 8,643 1 View
02 March 2021 3,060 3 View
28 February 2021 9,936 2 View
As a former management practitioner, I recall a generally accepted view that changing organisational culture is difficult, so you should only attempt it if the organisational change that you are...
27 February 2021 2,595 8 View
I diluted siRNA and RNAiMAX in opti-MEM and added to the cells which they were in the growth medium. Is it a right way? or should I culture cells in the opti-MEM medium for a while and not in...
26 February 2021 10,041 3 View
Hello, recently I am having some difficulties with maintaining the cell culture of primary cells isolated from a skin biopsy (normal and malignant). The coating of culture plates with Matrigel (a...
26 February 2021 6,151 2 View
Hi, We culture neural progenitor cells in T175 culture flasks coated with PLO-Laminin. We have successfully grown these cells with our protocol for months. Recently, we observed large sheets...
25 February 2021 3,443 4 View
I recently switched the company where we buy our DMEM/F12 and i have noticed a signficant change in growth for my human olfactory neurosphere cell lines
24 February 2021 2,268 3 View
Hi all, Can cells be reused after I have seed them onto 96 well plates and test them with cell counting kit at OD450 (CCK8) at 12 hours, wash them with PBS x3 and add new media and let them...
24 February 2021 5,072 1 View
Hello everyone, For part of my PhD project, I'm hoping to grow some lactobacillus species in the lab so that I can create standard curves for a qPCR quantification of a faecal sample. However, I...
24 February 2021 3,157 6 View
22 February 2021 9,932 11 View