Please let us know what works best in your hands.
Hello, I recently tried to get the mRNA sequence of WIP1/PPM1D gene from NCBI, but it does not contain uracils. Therefore I thought the listed mRNA sequence was actually a cDNA. So I used BLAST to...
30 June 2018 701 1 View
I am looking for answers from people who have directly compared the two variants of blood white cell telomere length analysis with phenotyping, i.e. the Baerlocher method, where the telomere probe...
29 January 2018 572 0 View
Sub-micrometer particles are usually measured by electron microscopy or by dynamic light scattering. Can a flow cytometer be used instead to distinguish between particle populations below 1...
11 January 2017 2,724 6 View
Where I could find the pedigree of Aubrac and some information about its creation?
13 February 2016 2,640 0 View
What is the possibility to obtain a fertile amphidiploid of Triticum sp. (e.g. Triticum dicoccum) and Henrardia persica?
18 January 2016 4,844 0 View
We look for bacteria specific antigens from the following species: Klebsiella pneumoniae, E.coli, Enterococcus faecalis, Proteus mirabilis, Pseudomonas aeruginosa Can anybody provide these?
31 March 2015 5,217 1 View
Can you recommend good and cheaper alternatives to FlowJo?
24 November 2014 7,440 7 View
We get only about 40% survival of our human PBMC stored at -150°C (in 90%FCS, 10% DMSO, Mr Frosty 1°C/min cooling in -80°C freezer). In contrast with human MSC, we have over 90% survival. Any...
02 November 2014 9,038 4 View
What could be the distribution (Normal, Poison) of the place of the optimized page in the Google search results, let's say.
11 December 2013 1,916 1 View
I will be grateful to hear about solutions which have worked in your own hands.
25 June 2013 1,682 5 View
What are the emerging trends and opportunities for STEM education and careers in the online space?
10 August 2024 507 0 View
Hello everyone, I recently submitted an article to Geotechnical Engineering (Proceedings of the ICE), and the current status has been listed as "EiC Pre-assessment: Ready" for the past 20 days. I...
10 August 2024 6,493 1 View
I have carried out MFC experiments on three different volumes, 50, 500 and 1000 mL of wastewater. Results after MFC treatment shows that TDS and EC are more in larger volumes of water i.e. TDS and...
09 August 2024 9,621 0 View
Hello everyone! I observed in my culture (htert-RPE1 cells) an orange- red particle at the bottom of the dish. It is visible to the naked eye as a very very small red dot. Could it be a...
09 August 2024 2,824 3 View
I am working in fungal fermentation of soybean meal and there is bacterial growth in them at times. I am trying to quantify fungal cell counts and bacterial cells; but I haven't been able to do at...
07 August 2024 7,535 4 View
Hi, I have a question about normalizing the MTT OD values for doing the statistical analysis. So, if we have 3 different plates and we call them 3 different replicates, so, first we would...
07 August 2024 8,106 4 View
Hello everyone, I have recently started using the microtome device for sectioning of paraffin-embedded mice lung. While I had some success in sectioning and observing proper ribbons, some of my...
06 August 2024 666 4 View
Previously when I co-coluture anti-CD19(FMC63) CAR-Jurkat with Raji with E:T=5:1, Jurkat can eliminate Raji in 24h. However, when I test another CAR construct, although I can dectect totally CD69...
06 August 2024 641 2 View
I will be with my students collecting seaweed samples in a marine farm and later we will process this tissue for RNA isolation and further sequencing. Does anyone have tips on how to collect the...
04 August 2024 501 2 View
I have tried several times to isolate lymphocytes from mouse spleen, but all attempts have been unsuccessful. I tried most available protocols. I used different dissociation media (HBSS with Ca...
04 August 2024 9,913 7 View
