Jivko V Stoyanov

4 Questions 34 Answers 0 Followers

Questions related from Jivko V Stoyanov

Can sub-micrometer particles be measured by a flow cytometer?

Sub-micrometer particles are usually measured by electron microscopy or by dynamic light scattering. Can a flow cytometer be used instead to distinguish between particle populations below 1...

12 January 2017 2,666 6 View

How can we get better survival of our human PBMC stored at -150°C?

We get only about 40% survival of our human PBMC stored at -150°C (in 90%FCS, 10% DMSO, Mr Frosty 1°C/min cooling in -80°C freezer). In contrast with human MSC, we have over 90% survival. Any...

03 November 2014 9,004 4 View

What would be a good battery of tests for detection of antibodies to extracellular matrix self-antigens?

I will be grateful to hear about solutions which have worked in your own hands.

26 June 2013 1,644 5 View

Differences in commercial DMEM F12 media - use in MSC differentiation

We have been using DMEM F12 for differentiation of mesenchymal stem cells and we presumed that different large commercial manufacturers stick to the same or very similar composition. Comparing the...

20 June 2013 6,610 9 View