Dear Pasupathi Sundharamoorthi: Oxaliplatin-resistant colon cancer cell lines SW620/L-OHP and lovo/L-OHP were established in vitro by continuous exposure to oxaliplatin (L-OHP) of low and gradually increased concentration by Lou et al (2010) and may be useful for you. Here is the paper reference:

Liu Z1, Qiu M, Tang QL, Liu M, Lang N, Bi F.    Establishment and biological characteristics of oxaliplatin-resistant human colon cancer cell lines.  Chin J Cancer. 2010 Jul;29(7):661-7

Chemotherapy is the main treatment for colon cancer, while multidrug-resistance is the main reason for chemotherapy failure and tumor relapse. This study was to establish two oxaliplatin-resistant colon cancer cell lines and evaluate their biological characteristics.

METHODS:

Oxaliplatin-resistant colon cancer cell lines SW620/L-OHP and lovo/L-OHP were established in vitro by continuous exposure to oxaliplatin (L-OHP) of low and gradually increased concentration. Growth curve, cross-resistance and resistance index of the oxaliplatin-resistant cell lines to various anti-cancer agents were determined by CCK8 assay. The expressions of P-glycoprotein (P-gp), multidrug-resistance protein 1 (MRP1) and MRP2 were detected by Western blot. Cell cycle distribution as well as the expression of CD133 and CD44 were measured by flow cytometry.

RESULTS:

It took 10 months to establish the SW620/L-OHP and LoVo/L-OHP cell lines with stable resistance to oxaliplatin. Cross-resistance to 5-fluorouracil, etoposide, cisplatin, vincristine and epirubicin but not to paclitaxel was observed. Longer doubling time, higher proportion of cells in G(0)/G(1) phase and lower proportion in G(2)/M phase were observed in the two oxaliplatin-resistant cell lines compared with their parental cell lines. The expression of MRP2 in the oxaliplatin-resistant cells was up-regulated, while those of P-gp and MRP1 had no significant change. CD133 was overexpressed while CD44 level remained unchanged in SW620/L-OHP and LoVo/L-OHP cells.

CONCLUSIONS:

SW620/L-OHP and LoVo/L-OHP cell lines show a typical and stably resistant phenotype and may be used as research model

Almost 20 years ago, researchers in NIH have done a study to screen the activity spectrum of oxaliplatin and other platin based chemoagents. For your question, you may find the answer in Fig.3 of the attached article.

Hi Yanqing Liu,

I am really appreciate your effort for finding reference...

Thank you very much...