8 Questions 21 Answers 0 Followers
Questions related from Pasupathi Sundharamoorthy
Hi friends, I am trying to determine ROS by FACS analysis. The protocol which I followed here, In this experiment I used colon cancer cell (HCT116) I used as a positive control H2O2 treated cells...
20 May 2016 8,938 4 View
I would like to know which cell lines are oxaliplatin resistance cell lines either colon cancer cell line or any other cancer cell lines. Thanks in Advance
16 September 2015 3,221 3 View
I have three compounds named as SMAL102, SMAL104 and SMAL104, the CMC value is 0.006, 0.040 and 0.2 mg/ml respectively. We analyzed the cytotoxicity in colon cancer and found SMAL102 showed...
16 July 2015 9,654 2 View
My compound increased PARylation (PAR) protein levels in time and-concentration depedent manner in HCT116 and HT-29 cells. Usually PAR activation induced AIF mediated cell death in neuronal cells...
15 May 2015 9,614 5 View
It's well know story Parylation(PAR) induced AIF mediated cell death. In my case i observed hyper activation of PARP induced parylation (PARP level was decreased), Parylated protein was higly...
24 March 2015 2,890 2 View
Universally apoptosis induces PARP cleavage (Full length 116kDa and cleaved PARP 89kDa). My compound induces PARP disappearance (similar to PARP inhibitors), so that my cell signalling antibody...
10 February 2015 2,520 8 View
I am doing RT-PCR for my experiment. I have CT value and after that I want to calculate fold difference between treatment and non-treatment. If somebody has an Excel template, could you send to...
25 November 2014 6,116 10 View
My compound induced apoptosis proved by annexin v staining, in the case of western blot experiment I was trying to prove through caspase and PARP cleavage. I could not absorb caspase-3, 8 & 9...
19 October 2014 6,783 12 View
