Is there a RT-PCR quantification Excel template?

More Pasupathi Sundharamoorthy's questions See All

ROS determination by FACS?

Hi friends, I am trying to determine ROS by FACS analysis. The protocol which I followed here, In this experiment I used colon cancer cell (HCT116) I used as a positive control H2O2 treated cells...

19 May 2016 8,919 4 View

Which cell lines are Oxaliplatin resistance cell line?

I would like to know which cell lines are oxaliplatin resistance cell lines either colon cancer cell line or any other cancer cell lines. Thanks in Advance

15 September 2015 3,203 3 View

What is the importance of CMC(Critical micelle Concentration) for anti-cancer compounds?

I have three compounds named as SMAL102, SMAL104 and SMAL104, the CMC value is 0.006, 0.040 and 0.2 mg/ml respectively. We analyzed the cytotoxicity in colon cancer and found SMAL102 showed...

15 July 2015 9,631 2 View

Does PARylation cause cell death in colon cancer cell?

My compound increased PARylation (PAR) protein levels in time and-concentration depedent manner in HCT116 and HT-29 cells. Usually PAR activation induced AIF mediated cell death in neuronal cells...

14 May 2015 9,592 5 View

Any suggestions on Parylation(PARP) induced AIF mediated cell death?

It's well know story Parylation(PAR) induced AIF mediated cell death. In my case i observed hyper activation of PARP induced parylation (PARP level was decreased), Parylated protein was higly...

23 March 2015 2,869 2 View

Is there any drug which can have self assembling properity?

I have one synthetic drug which have two property 1. it can be used as a anti cancer drug. as well as 2. it can be used as a drug carrier. I could not able to find any reference like self...

29 January 2015 3,139 2 View

How can I prove caspase-independent cell death?

My compound induced apoptosis proved by annexin v staining, in the case of western blot experiment I was trying to prove through caspase and PARP cleavage. I could not absorb caspase-3, 8 & 9...

18 October 2014 6,764 12 View

How to learn more about SPSS and its Application?

I would like to learn more about SPSS and Its application especially in regards to data analysis. Please suggest me how I can learn more about it. Thank you so much.

11 August 2024 9,101 4 View

Can I base on reverse DNA sequences to perform alignment, convert to amino acids and GenBank submission?

I have reverse sequences (AB1 format), can I base on reverse DNA sequences to perform nucleotide alignment, convert nucleotides to amino acids and deposit the sequence in GenBank database?

11 August 2024 5,138 1 View

Baseline drift in HPLC? What causes this?

Hello, Why do i see this baseline drift when i compare my blank (black) to the sample (blue)? Any suggestions as to why this happened? Thank you!

11 August 2024 3,770 4 View

Text-Communication from the M1 Hand Area using BCI—and then there is Elon Musk?

Willett, Shenoy et al. (2021) have developed a brain computer interface (BCI) that used neural signal collected from the hand area of the motor cortex (area M1) of a paralyzed patient. The...

10 August 2024 7,180 0 View

Handling Missing Data and Building a Predictive Model with Incomplete Information ?

I am developing a predictive model for a water supply network that involves 20 influencing points. However, I only have historical data for 10 out of these 20 points. I would like to know how to...

10 August 2024 4,005 2 View

Has anyone applied Python in the field of textile engineering for data analysis, automation, or smart textiles?

I'm currently exploring the application of Python in textile engineering, specifically in areas like data analysis, process automation, and the development of smart textiles. I'm interested in...

10 August 2024 7,429 2 View

How can I use the cif data obtained from rietveld refinement extracted via gsas2, for microstructural analysis using ETEX software?

09 August 2024 7,718 0 View

How to confirm the site-directed mutagenesis result without performing NGS?

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08 August 2024 4,645 2 View

I can't see the ssDNA band after performing asymmetric PCR. Is there any way to do this?

After performing symmetric PCR, PCR purification was performed. Afterwards, asymmetric PCR was performed using the PCR purification product as a template, but no ssDNA band was confirmed in the...

08 August 2024 1,668 3 View

How are iso-frequency contours plotted?

Let's say we have a standard, regular hexagonal honeycomb with a 3-arm primitive unit cell (something like the figure attached; the figure is only representative and not drawn to scale). The...

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Gaurav Nagar Popular answer

i have one such template. i hope it helps.