Which cell line offers the highest transfection efficiency? in Multiple myeloma or Neuroblastoma. I heard in multiple myeloma RPMI-8226 and U266 is hard to transfect.
and Neuroblastoma IMR-32 is low transfection efficiency. Is there other cell line to easily transfection? in multiple myeloma or neuroblastoma.
Hello
B35 and B50 neuroblastoma cell lines are easy to transfect.
Please refer to the research article below for more information.
doi : 10.1016/s0091-679x(03)01013-6
Best Wishes.
Dear Yoo Dong!
SK-N-AS neuroblastoma cell line has Transfection Efficiency at least 90% for siRNA delivery. Transfection efficiency was determined by qRT-PCR.
https://altogen.com/product/sknas-transfection-reagent-neuroblastoma-cells/
Article Efficient Transient Transfection of Human Multiple Myeloma C...
Table 1
Parameters for MM cell line electroporation.
Article Transfection of siRNAs in Multiple Myeloma Cell Lines
(attfched)Fig. 2 Negative control Alexa 488-labelled siRNA transfection into myeloma cell lines using the Streptolysin-O reversible permeabilization method. RPMI8226 and U266 cells were treated with 8 and 10 Units of activated Streptolysin-O, respectively. Transfection was performed in the presence of 5 µM of siRNA duplex. Transfection efficiency was assessed 24 h later using FACS. The use of activated Streptolysin-O induced a substantial uptake of the siRNA duplex by the myeloma cells above 80%. (Reproduced from ref. 8 with permission from Elsevier Science)
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