Transfection efficiency depends on a lot of factors among which the ''the cell line type'' is one of them? In general, which cell line shows maximum transfection efficiency and how much?
Hello Puneeta
The transfection efficiency really depends of what you are intending to express and in which system. If you are interested in mammalian cell lines, HEK 293T and HeLa have very good transfection efficiencies even via transient transfection modalities.
I would really pay attention to what transgene you would like expressed, for eg: if its a toxic protein or a protein of very distant origin, you may need to consult the best cell line by testing several.
Hope this helps
Aparajita
Hela, Hela EG, MDA-MB-231, HUVEC ; CHO, are easy to transfect
Hi Puneeta,
I highly agree with Aparajita; it is better to look at the objective(s) of your study before deciding which cell to use. Anyway, I think HEK293 is very easy to work with and achieve a transfection efficiency of more than 95 %.
Good luck with your experiment!
Radhwan
Hi Puneeta,
I agree with Aparajita and Radhwan; you have to chose suitable cell line according to the experiment you are intending for. However, in my own experience Hek 293, HeLa, and H1299 are very easy to transfect. I have worked with Hek 293 and H1299 and they get transfected easily.
Would just like to add few points b4 u proceed for the actual transfection
1) Do transfection when your cells have about 70% or more confluency
2) Mostly you should do transfection in the cell lines with passage no as low as 2 or 3 and as high as 20, 25 not more than that because of the subtle alteration in their genetic makeup as they undergo passages.
3)The choice of transfection reagent viz. lipofectamine 3000 (InVitrogen) or your own lab made transfection reagent.
4) Just as i was going through i found a research article in relevance to your question. Here is the attached file.
Good Luck and shubhkamnaye!!
Thanks Vikrant Mehta Radhwan Al-Zidan Aparajita Lahree Vikrant Mehta ! Your inputs are quite useful.
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