20 Questions 13 Answers 0 Followers
Questions related from Puneeta Bathla
We all know that digestive enzymes speed up the chemical reaction and helps in food digestion. I was wondering if in general it takes 6 h to digest a food with the help of digestive enzymes then...
05 October 2019 5,604 3 View
I recently coated the 96 well plates with 0.1 % w/v (1:10) dilution of Poly-L-Lysine for seeding the HEK-293T cells. However, I found that the attachment of the cells to the surface is not...
25 May 2019 424 2 View
I have to perform fluorescence based assays in 96-well plate using HEK-293 cells. The experimental procedure involves transfection in cells and then treatment with drugs for testing drug efficacy....
02 May 2019 3,702 9 View
Enzymes are synthesised as zymogens and then make a transition to active form when required, is this true for every enzyme or are there any exceptions to that?
18 March 2019 4,454 3 View
I have purified a monomeric EGFP- His protein with a molecular weight of 30 kDa. But, instead of 30 kDa I am getting a thick band of 60 kDa in SDS-PAGE reducing gel. what is the possible reason...
31 January 2019 5,865 2 View
I have purified a EGFP-His tagged protein which was there in pET15b vector using Ni-NTA column. The concentration obtained was way too high around 5mg/ml. Before the running the SDS-PAGE the...
31 January 2019 7,341 2 View
I have a vector pET23b with monomeric EGFP-6X His tagged cloned in that. The plasmid is transformed in BL21(DE3) cells. Kindly suggest me some good literature for the purification of the GFP-His...
12 January 2019 2,482 4 View
Best buffer composition for storing a recombinantly expressed and purified mEGFP protein ?
12 January 2019 8,749 4 View
Have to see protein-protein interaction in HEK-293 cells, where the donor GFP tagged protein and acceptor is RFP tagged protein. Please suggest me some good tips that I should keep in mind for...
12 January 2019 7,133 1 View
Transfection efficiency depends on a lot of factors among which the ''the cell line type'' is one of them? In general, which cell line shows maximum transfection efficiency and how much?
03 January 2019 2,863 5 View
FLAG tag the octapeptide sequence and artificial epitope binds to the FLAG antibody. I want to know in which region of the FLAG antibody does the FLAG epitope binds ?
21 September 2018 6,154 2 View
I have a protein of molecular mass 400 kDa? What is the required protein gel concentration for both stacking and resolving gel?
03 April 2018 2,753 1 View
I have to perform an intracellular FRET assay in 96 well plate and looking for a nice protocol in this regard.
17 March 2018 5,267 1 View
I've got a plasmid from SinoBiological- Casp-3 GFPspark cDNA clone. In the section of expression method it says- Constitutive, Stable/transient. I would like to know what ''stable'' is here? I...
09 March 2018 6,833 2 View
I would like to know if anyone has created a stable cell-line of MCF-7 cells expressing Caspase-3 GFP gene?
09 March 2018 8,769 1 View
I want to know some useful tips which help me in doing transfection in 96 well plate ?
24 February 2018 7,002 3 View
Nuclear stain like DAPI and Hoechst stains nuclei and also reveals the presence of mycoplasma. So, my question is does PI stain along with staining the nucleus tell us the presence of mycoplasma?
15 January 2018 5,560 2 View
I made a stock solution of 500uM for Z-VAD-FMK caspase inhibitor using DMSO solvent, however, I now realize that I need a stock of either 1or 5mM. Is there any trick to evaporate DMSO solvent in...
10 January 2018 3,390 2 View
Studying necroptosis pathway
28 December 2017 3,349 2 View
MCF-7 cell line is caspase-3 null that means it doesn't express caspase-3 and hence a suitable model for studying the function of caspase-3 by exogenous expression tagging it with fluorescent...
19 December 2017 8,397 3 View
