What might be the reason for obtaining two bands in plant genomic DNA extraction?

I got a thin band and a thick band i guess it would be the genomic DNA and the 260/280 ratio is 3.

21 February 2021 6,523 6 View

Any recommendations for tetR antibody for IHC?

I am planning an experiment where I've to detect cells which are expressing TetR, my vector is so big that I can't have a reporter in it. I was thinking if I can detect the expression of TetR...

24 September 2020 1,041 4 View

How do I reduce the size of a file in Comsol simulations?

I am working with RF module in comsol and doing parametric sweeps in frequency domain. The data generated is huge and taking a lot of space on my disc. The fine meshing that I need for my problem...

22 July 2020 4,374 6 View

How to get the antenna to resonate at the required frequency with the ideal magnitude in the s-parameter graph?

I have designed a rectangular patch antenna for a frequency of 2.4Ghz using Rogers 5880 as my substrate material with a thickness of 1.6mm. But, my s-parameter graph does not show a graph with the...

03 October 2019 6,543 4 View

Best reagent for Colorimetric detection of chloride ions?

I want to find an assay for colorimetric method of detection of chloride ions from blood or body fluids. The main issue is I cannot use mercury or mercury derived chemicals in my assay. Can...

04 February 2019 8,093 3 View

How to grow N.gonnorhea?

02 August 2016 1,264 1 View

I tried extracting plasmid DNA by Brimbroim Dolly method. After running on agarose gel I am getting mutiple bands of different molecular weights.why?

Are all the bands plasmids itself?or is it degraded plasmid?

30 July 2016 7,069 11 View

What can be the possible outgroup for Ryphilla sp (crab, leucosidae) while drawing phylogenetic tree with Co1 gene??

Which all organisms can be chosen as best outgroups for Ryphilla crabs in the phylogenetic tree

30 July 2016 9,087 1 View

Can you provide any method for APEO analysis on HPLC?

bufffer of what pH can we use as mobile phase?

01 March 2016 8,861 1 View

Can anyone please send me the procedure for plasmid extraction from Vibrio species?

Can anyone please send me the procedure for plasmid extraction from Vibrio species?

11 February 2016 1,768 2 View

What SPSS analysis should I run with one IV (with two levels) and two DVs?

Hi, I am trying to figure out which test would be most appropriate to run for the following question: Is there a difference in task blood pressure between smokers and non-smokers? There are two...

01 March 2021 8,235 2 View

Ester cleavage conditions?

Hi all, I need to cleave the ester in Ethyl 2-amino-1H-imidazole-5-carboxylate to make the carboxylic acid and retrieve the product from solution to use in downstream applications of linking to...

01 March 2021 8,766 3 View

Expression cloning by using cDNA,do I need to modify the cDNA first?

I am going to have a expression cloning of mammalian gene by using shuttle plasmid to transforming the E.coli However I don't know I should only inserting the Coding sequence ,or I can...

28 February 2021 5,440 3 View

Mediation path analysis - Can I run two seperate models?

I am wanting to run a serial mediation model with 2 x IVs, 2 x MVs, and 3 DPs (all continuous). Is it problematic to run 2 different models for each IV, keeping the MVs and DPs the same in each...

27 February 2021 5,681 3 View

SEM or Multiple Regression for an analysis without Latent Variables?

Hello, I have three hypothesis to test, all with the same IV. I am testing if the effect of the IV on the first DV is mediated by awe. I am then measuring whether the effect of the IV on a...

26 February 2021 8,399 5 View

Why do the PCR bands from the cDNA templates (extraction from the cell after transfection) show different size from the band of the plasmid template?

After transfection with the plasmid ( linearized ) and subcloning of the cell lines, RNA was extracted from the cell and then reverse transcripted to cDNA. When PCR reactions were run to verify...

25 February 2021 5,712 3 View

Are there lab markers/pens resistant to Roti-Histol?

Hi, I'm looking for a pen/marker to write on the smooth, transparent (non-frosted) part of microscopy slides which does not get washed away by alcohols and most importantly Roti-Histol. I want to...

24 February 2021 9,902 5 View

What is the best concentration of cDNA to be used for qPCR?

I have synthesised cDNA from 500ng/ul of RNA. The concentration of the cDNA Is about 5000ng/ul. Should I dilute my cDNA or just use it as it is for my qPCR? I usually use 2ul of cDNA for a 20ul...

24 February 2021 8,618 3 View

Is it efficient to do liquid biopsy to investigate the gene expressions of the tumor?

To do liquid biopsy and look for specific gene expressions there are so many published papers. Thera are different protocols and procedures used to isolate circulating cell free RNA or mRNA from...

23 February 2021 7,326 5 View

QPCR reaction efficiency with odd results what does this indicate?

I am working on a CFX 96 machine to determine reaction efficiency of my gene of interest. After performing a serial dilution of my cDNA template, I was able to find great reaction efficiencies for...

23 February 2021 809 3 View