Which cDNA synthesis kit would you suggest; Superscript VILO cDNA synthesis or Superscript IV cDNA synthesis (Invitrogen) ?

More Reshma Tuladhar's questions See All

What are the methods for analyzing trihalomethanes in water other than gas chromatography and liquid chromatography?

for monitoring disinfection byproducts in drinking water

12 October 2023 8,613 3 View

I am doing base hydrolysis of ester with LiOH and THF:H2O( 1:1) but after hydrolysis in acid NMR I am getting 4 proton extra peak?

4 protons peak may be CH2 near a heteroatom .

13 July 2023 1,075 1 View

How to reduce air bubble formation in film?

The film forming solution (FFS) is stirred overnight and it is oven dried at 40°C. Please give me suggestions to reduce the air bubble.

09 July 2023 7,857 4 View

Does anyone know how to prevent protein coeluting with other impurities in size exclusion Column?

I have been trying to purify a transcription factor. Protein has MBP and His Tag. I use His TRAP as my first purification step and then after I do size exclusion, but my protein (83 KDa) elutes...

23 April 2023 9,182 5 View

How to recover the Pd(PPh3)4 catalyst, already become dark brown?

How to store this catalyst?

06 March 2023 9,026 1 View

How do I determine doping concentration of a new element in Barium Titanate structure if I want to produce a new system?

I want to produce a new system of Barium Titanate and characterize it for high dielectric constant values at microwave frequencies.

02 February 2023 4,203 8 View

How to find Yaw joint angle of differential drive robot ?

I'm designing leader follower feedback controller for crawler robots .Here rear wheel is connected to front via a joint so that it can follow front when it turns ,for both the module(front...

20 December 2021 1,203 3 View

Multivesicular liposomes formulation?

multivesicular liposomes are prepared by double emulsification method. Can someone tell how to evaporate organic solvent from multivesicular liposomes preparation without emulsion cracking?

04 March 2021 5,166 3 View

What might be the reason for obtaining two bands in plant genomic DNA extraction?

I got a thin band and a thick band i guess it would be the genomic DNA and the 260/280 ratio is 3.

21 February 2021 6,871 6 View

Any recommendations for tetR antibody for IHC?

I am planning an experiment where I've to detect cells which are expressing TetR, my vector is so big that I can't have a reporter in it. I was thinking if I can detect the expression of TetR...

24 September 2020 1,293 4 View

Why activated CAR-Jurkat cell could not kill targets?

Previously when I co-coluture anti-CD19(FMC63) CAR-Jurkat with Raji with E:T=5:1, Jurkat can eliminate Raji in 24h. However, when I test another CAR construct, although I can dectect totally CD69...

06 August 2024 641 2 View

Can I please ask why my samples from anaerobic bioreactor giving me different size PCR product even after multiple runs?

Hi everyone, I have extracted DNA from a biogas bioreactor using Qiagen kit and prep cDNA library then used this library as template to optimize primers for qPCR (taken from papers). Some of the...

23 July 2024 1,329 5 View

I want to buy Hydrothermal Synthesis Autoclave from any European company. Can anyone suggest any company inside Europe?

Thanks

22 July 2024 1,143 3 View

Hi dear researchers, do you suggest the best and shortest path for chemical synthesis and green synthesis of magnetite(Fe3O4)?

Suggest the best and shortest route for the chemical synthesis and and green synthesis of magnetite(Fe3O4) The chemical synthesis should contain the least dangerous chemicals along with full...

21 July 2024 2,476 2 View

What is the suitable solvent of MIL-100 for chemotherapy?

I synthesised a nanocarrier by conjugation MOF (MIL-100) with folic acid for use in chemotherapy but the samples didn't dissolve completely in aqueous solution or DMSO. Instead, they only...

19 July 2024 8,522 0 View

You have mentioned as a co-author of one publicaitron?

You have mentioned as a co-author of one publication, but it is not exactly a publication , it is an abstract submitted to: Congresso Brasileiro de Hematologia, Hemoterapia e Terapia Celular -...

17 July 2024 4,035 15 View

Im trying iodination of an arene, but only getting black precipitates, is there anyone with iodination experience who can advise?

Organic Synthesis. My student has been trying iodination of arenes (our goal is to iodinate p-phenylenediamine) following these literature procedures: 1. Synthesis 2004, No. 11, 1869-1873 using...

16 July 2024 6,630 1 View

What could be the possible reason that sintering of polycrystalline diamond fabricated with tape casting has suddenly started failing?

I make polycrystalline diamond with tape casting followed by HPHT sintering. When i sintered the samples up till May, the sintering was good. When i sintered the samples in June and July at the...

16 July 2024 3,212 5 View

What is the suitable solvent of MIL-100 for chemotherapy?

I synthesised a nanocarrier by conjugation MOF (MIL-100) with folic acid for use in chemotherapy but the samples didn't dissolve completely in aqueous solution or DMSO. Instead, they only...

15 July 2024 2,414 1 View

Can I use DNA a ladder for RNA samples in agarose electrophoresis? Or It is preferable to use cDNA amplified from RT-qPCR for the same samples?

I performed a nucleic acid extraction using 2 protocols for RNA extraction (Dengue Virus) and one parameter I need to evaluate is the integrity of my RNA samples. In this case, I can't use a RNA...

09 July 2024 4,857 2 View