What might be the reason for obtaining two bands in plant genomic DNA extraction?

I got a thin band and a thick band i guess it would be the genomic DNA and the 260/280 ratio is 3.

21 February 2021 6,523 6 View

Any recommendations for tetR antibody for IHC?

I am planning an experiment where I've to detect cells which are expressing TetR, my vector is so big that I can't have a reporter in it. I was thinking if I can detect the expression of TetR...

24 September 2020 1,041 4 View

How do I reduce the size of a file in Comsol simulations?

I am working with RF module in comsol and doing parametric sweeps in frequency domain. The data generated is huge and taking a lot of space on my disc. The fine meshing that I need for my problem...

22 July 2020 4,374 6 View

How to get the antenna to resonate at the required frequency with the ideal magnitude in the s-parameter graph?

I have designed a rectangular patch antenna for a frequency of 2.4Ghz using Rogers 5880 as my substrate material with a thickness of 1.6mm. But, my s-parameter graph does not show a graph with the...

03 October 2019 6,543 4 View

Best reagent for Colorimetric detection of chloride ions?

I want to find an assay for colorimetric method of detection of chloride ions from blood or body fluids. The main issue is I cannot use mercury or mercury derived chemicals in my assay. Can...

04 February 2019 8,093 3 View

How to grow N.gonnorhea?

02 August 2016 1,264 1 View

I tried extracting plasmid DNA by Brimbroim Dolly method. After running on agarose gel I am getting mutiple bands of different molecular weights.why?

Are all the bands plasmids itself?or is it degraded plasmid?

30 July 2016 7,069 11 View

What can be the possible outgroup for Ryphilla sp (crab, leucosidae) while drawing phylogenetic tree with Co1 gene??

Which all organisms can be chosen as best outgroups for Ryphilla crabs in the phylogenetic tree

30 July 2016 9,087 1 View

Can you provide any method for APEO analysis on HPLC?

bufffer of what pH can we use as mobile phase?

01 March 2016 8,861 1 View

Can anyone please send me the procedure for plasmid extraction from Vibrio species?

Can anyone please send me the procedure for plasmid extraction from Vibrio species?

11 February 2016 1,768 2 View

Polymer composites suddenly solidifies?

I am making polymer composite by solvent mixing method. When I am trying to add filler solution in polymer solution it quickly get solidified. How to make them then uinform? It doesn't give time...

03 March 2021 7,540 4 View

What solvent can I used for Paal Knorr reaction between 6-amino hexanoic acid and 2.5 hexanedione?

I am try to make the Paal Knorr reaction between 2.5 hexnedione and 6-amino hexanoic acid, my problem is type of solvent to use, because 6-hexaminohexanoic acid is soluble in water but I am not...

02 March 2021 4,443 2 View

How to polymerize TEOS?

I want to polymerize TEOS on a surface. I know that NH4OH is a preferred catalyst and an alcoholic solvent should be used .But I can not find certain amounts of each material to use. Does any one...

02 March 2021 1,404 6 View

How can extract ciprofloxacin hydrochloride disolve in water er to organic solvent such as dichloromethan or any other solvent?

I need a procedure to extract the ciprofloxacin hydrochloride from aqueous solution by using dichloromethane in detail,. I thank you very much.

01 March 2021 4,306 1 View

How to separate water from glycerol solvent in a solvent recovery vacuum distillation column?

I am currently doing my third year design project. I have been assigned to design a glycerol recovery column in vinyl acetate monomer production. The process unit before the recovery column is an...

01 March 2021 9,525 4 View

How to remove fatty material from plant extract?

The resultant solid was partitioned three times with 250 ml of petroleum ether (40-60/c) to remove any fatty material and evaporated to dryness.

28 February 2021 9,464 2 View

1 nm thick MgO thin-film to be deposited on Si wafer using thermal evaporation. What will be the optimum deposition conditions?

Favourable conditions of base pressure, rate of deposition. Also, by which characterisation technique to be used to verify the thickness of the deposited film.

28 February 2021 3,640 3 View

How to make fractions of chloroform extract of plant, using seperating funnel?

28 February 2021 8,466 2 View

Why am i not getting solid after reacting APTES with salicylaldehyde?

The reaction of APTES with salicylaldehyde in ethanol leads to the formation of imine compound yellow solid which is mention in literature, but I am not getting yellow solid I don't know why? when...

28 February 2021 2,955 2 View

can I treat waste water containing solvents and alcohol with electrocoagulation?

there are solvents and alcohol in the waste water of a factory that produces disinfectants. Increasing this waste water requires very serious operations. Can I treat this waste water with...

27 February 2021 2,609 3 View