To know polarity defect (by knocking out some specific genes) I am looking for a breast cancer cell line that has high polarity, forms tight junctions and easy to transfect.
Although I don't know of a breast cancer cell line that would ideally match your characteristics, I would look into MDA-MB-231 and MCF-7. Both are highly characterized (MDA-MB-231 I believe is polar Article Untargeted polar metabolomics of transformed MDA-MB-231 brea...
), and both are known to form tight junctions (Article Hepatocyte growth factor disrupts tight junctions in human b...
). Both cell lines are likewise easy to transfect (see this reagent for MDA-MB-231: https://altogen.com/product/mda-mb-transfection-reagent-breast-adenocarcinoma/ and this one for MCF-7: https://altogen.com/product/mcf-7-transfection-reagent-breast-cancer-htb-22/). There are probably other cell lines that might better work for your research, but I wouldn't know which.
Although I don't know of a breast cancer cell line that would ideally match your characteristics, I would look into MDA-MB-231 and MCF-7. Both are highly characterized (MDA-MB-231 I believe is polar Article Untargeted polar metabolomics of transformed MDA-MB-231 brea...
), and both are known to form tight junctions (Article Hepatocyte growth factor disrupts tight junctions in human b...
). Both cell lines are likewise easy to transfect (see this reagent for MDA-MB-231: https://altogen.com/product/mda-mb-transfection-reagent-breast-adenocarcinoma/ and this one for MCF-7: https://altogen.com/product/mcf-7-transfection-reagent-breast-cancer-htb-22/). There are probably other cell lines that might better work for your research, but I wouldn't know which.
I do not believe that such a cell line exists fulfilling the criteria forming a restrictive monolayer of polarized cells akin to MDCK, Caco-2, etc.
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