I need help with mflab software and aquifer modeling?

I'm trying to model an aquifer using the codes in SWI folder of the software. However, I have encountered a problem. The codes and the corresponding excel files have illustrated the situation...

05 June 2019 1,449 2 View

How much would it cost to build a CRISPR/Cas9 system using macrophages cell line? What machines and raw materials are needed?

How long it will take for a successful editing?

31 December 2018 5,741 1 View

Which breast cell line makes baso-lateral membrane,tight junctions and has high polarity?

To know polarity defect (by knocking out some specific genes) I am looking for a breast cancer cell line that has high polarity, forms tight junctions and easy to transfect.

25 July 2018 9,592 3 View

How much chlorine is present in 5.75% NaOCl. How to calculate it?

I am anlyzing bleaching powder sample (5.75%) through titration which gives the amount of chlorine present. How to convert chlorine content into NaOCl?

11 December 2017 1,566 0 View

While I was using WB, I found lots of scattered band where I never expected. What is the reason?

01 February 2016 3,249 4 View

Why did my cells disconnected during the immunofluorecence procedure?

I am trying to do IF to U2OS cell line in 96 wells plate. during the procedure I lose many cells which dissociate off the plate. Does anyone has a tip how to stay with many cells at the end of the...

28 June 2015 6,856 4 View

Which is an appropriate solvent for melatonin?

04 May 2015 1,869 14 View

Which solvent can I use to dissolve melatonin for the macrophage cell line?

I want to work with the macrophage cell line with melatonin. Which solvent and how can I use it for dissolving melatonin minimizing solvents adverse effects? Can you answer elaborately with its...

04 February 2015 9,889 3 View

Which solvent can I use to dissolve melatonin for the macrophage cell line?

I want to work with the macrophage cell line with melatonin. Which solvent and how can I use it for dissolving melatonin minimizing solvents adverse effects? Can you answer elaborately with its...

01 February 2015 2,685 4 View

How can I detect or measure several proteins or mRNA by a single method simultaneously?

I would like to measure the expression of several proteins or mRNA simultaneously. I mean, can I measure by one nitrocellulose membrane (as all of my target proteins or mRNA will be present there)...

01 February 2015 9,842 6 View

Tooth extraction and subsequent dental implant placement in Sprague-Dawley rats induce differential changes in anterior digastric myofibre size and ?

01 March 2021 7,254 3 View

What are the pros and cons for mice versus rats?

Hi Everyone, I am designing a pilot study using rodents to assess the effects of simulated space environments (radiation + altered gravity) on microbiome and immune system. I have limited...

24 February 2021 1,825 3 View

Experimental Design: How deal with pseudreplicates with immunological and clinical-chemical paramaeters?

Hello everybody, I am investigating the influence of a toxinon various clinical-chemical and haematological parameters in fish. I have three treatments (control, low dose, high dose) in my...

24 February 2021 1,243 1 View

What should be the duration of exposure to simulated space environments with Rodents?

Hi, I am currently working on a pilot study to get some data related to the impact of long duration spaceflight to the microbiome and the immune system. I plan to use rats and simulate...

22 February 2021 592 1 View

Which way will be better to give OGT inhibitors doses through intravenous or orally for pregnant female rats and concentration suggestion?

I'm planning to use ST078925 OGT inhibitor from TimTec (OGT-O-linked N-acetylglucosamine (GlcNAc) transferase) for female rats.

16 February 2021 7,850 2 View

How can you tell if your organotypic slice culture is healthy ?

I'm working on organotypic cerebellar slice cultures from postnatal rats and I'm not sure how to tell if the slices are healthy at any given point. I know there should be a lot of microglial...

15 February 2021 2,830 2 View

Why daily dexamethasone given to rats fed high fat/fructose/salt cause very high mortality rate?

rats were given daily dexamethasone in drinking water corresponding to 0.5 mg/kg or 2 mg/kg. These rats are simultaneously fed 25% fat, 3% salt in diet and 10% fructose in drinking water but...

15 February 2021 7,634 2 View

How to calculate the sample size required to detect the difference in pharmacokinetics of methotrexate?

I came across with this study here, https://www.clinexprheumatol.org/abstract.asp?a=5066 And this study says: "The sample size calculation was done to compare two different MTX starting dose...

14 February 2021 3,017 3 View

Can anyone suggest a way to dissolve insulin which is for rat/mouse injection?

We are trying to inject insulin to rat or mouse, but the insulin we've got is powder, and there are many ways for dissolve, like in saline, PBS, DMSO, or in Tris-HCl, can anyone give us a...

13 February 2021 8,426 10 View

How to calculate ultraviolet dose in Gy or J/kg?

Currently I'm doing a literature review regarding the comparisons of different types of radiation such as uv, electron beam and gamma radiation. But I'm having a difficult time converting uv unit...

11 February 2021 7,642 3 View