Twenty one rats received 1.2 mg/kg A substance (MW:294.98 g/mol) dissolved in 1% Tween 80 by oral gavage for 14 consecutive days. How should I dilute substance A? How can I calculate the dose to give to rats? Can you help with this issue?
I have tried to achieve a separation of the nucleus and cytoplasm by using RLN buffer+ NP40 (0.2%), and then proceeding with GeneMATRIX universal RNA purification kit (the same used for total RNA...
04 February 2024 7,172 2 View
Hello all, In my research I intend to purify ChlG (a membrane protein) from cyanobacteria using a protein tag. I want to elute the protein together with its interacting partners or complexes. I...
26 October 2023 3,119 1 View
I am looking into modeling photoresist thermal reflow processes and looking for a mathematical model that can give me the effect of time on the reflow process (thickness, curvature, ect.). Thank you!
13 August 2023 3,378 0 View
I have multiple thin layers on my substrate and I want to use HMDS to improve the surface tension before using photoresist. The issue is that the developer solution will remove it and for the next...
10 April 2023 3,432 2 View
Hello to you, I would like to know if someone here can explain to me the difference between the following models for urban microclimate analysis before I start researching each one myself: -...
10 October 2020 3,192 3 View
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05 April 2020 8,653 12 View
I conducted an I-V curve measurement for my SWCNT FET and received a very odd result that I can't understand. The measurement was done with DI water as gate dielectric under gate voltage between...
25 August 2019 1,958 2 View
As you know, in accordance with the first law of switching, the current in the branch with an inductive coil, and in accordance with the second law of switching, the voltage across the capacitor...
09 August 2019 7,689 17 View
I'm trying to model an aquifer using the codes in SWI folder of the software. However, I have encountered a problem. The codes and the corresponding excel files have illustrated the situation...
05 June 2019 1,478 2 View
do someone have a 2D coastal aquifer model of rectangular shape or other shapes , with the basic input parameters (including some wells with location and rate of pumping) for numerical models.?...
30 May 2019 9,583 6 View
I did PR spin coating on trench structure. I used AZ P4620 PR and the thickness or PR is around 11um. The substrate is Si. And my trench structure depth is 141um(negative way). Even though I...
08 August 2024 2,298 0 View
Hi all, I was just wondering if anyone has experience with multiplexing a mouse monoclonal primary and a rat primary. I'm trying to multiplex by incubating them in the same well but was told by a...
06 August 2024 9,710 1 View
I´ve been unable to find specific information about this neurotrophin in the CNS of rabbits exclusively. There is extensive info in mice, fish and rats, but in brain´s rabbit is hard to find....
04 August 2024 762 1 View
Reference dose and Maximum acceptable concentrations HMs
03 August 2024 8,230 4 View
Dear All I would like to know the minimum number of animals (rats) to be studied for a pilot/preliminary research on intradermal drug delivery. Can I proceed with 1 animal per group for a pilot work?
02 August 2024 4,570 1 View
I used humic acid at 0.044 g/kg soil in my pot experiment. But finally, I have to recommend kg/ha. Each pot's soil weight was 11 kg. What is the solution?
02 August 2024 7,186 6 View
Hello I am trying to create a stable cell line in HEK293 via Lipofectamine 3000 transfection. My plasmid is a CD63-IL10-GFP construct with Puromycin resistance. I am successful with the...
30 July 2024 6,648 1 View
Dear researchers. I tried using the IHC PROFILER in image j to quantify nuclear DAB staining. I followed the instructions in the original article by "Varghese F, Bukhari AB, Malhotra R, De A...
29 July 2024 2,229 0 View
Dear all, I am working on particle deposition in human's & rat's respiratory airways using CFD and I am looking for the 3D CAD file for my simulations (STEP or IGES format). If somone has such...
29 July 2024 1,092 2 View
i compare between the antioxidant and oxidative stress level between negative control rats (saline) and CCL4 intoxicated rats. i found the level of GSH and TAC is more in toxic group than normal....
26 July 2024 1,887 0 View