Which are the small factors that leads to DNA degradation by manual method?

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Is there any criteria to differentiate between crystalline and amorphous peak in XRD for polymers ?

can anyone suggest me the book to know the answer to the above question? the graph has been attached

07 September 2020 7,512 4 View

How to calculate the P(0) in the RLS method with adaptive forgetting factor?

the Root least square method with forgetting factor for implementation in MATLAB i have an ARX model for parameter estimation. How to calculate the P(0) for this method ?

15 June 2020 2,168 2 View

Is it possible to derive sample size using ROC curves?

I have a dataset containing 130patients who underwent surgery. I was determining the effect of intraop blood transfusion on post-op respiratory complications and NIV use. ROC for transfusion Vs...

27 March 2020 1,011 3 View

Which standard method can be used for quantification of nitrobenzene in aqueous phase?

Nitrobenzene is sparingly soluble in water. I tried plotting its standard curve with various dilutions ranging from 0.1mM - 1mM, using HPLC with the method given in papers (Mobile phase: Methanol...

17 March 2020 5,306 3 View

Is there any good journals which publish fuzzy group papers without fee?

Please provide some free SCI/SCIE/SCOPUS indexed journal for fuzzy group.

30 December 2019 5,952 1 View

Where can i get the properties of exhaust gases at high pressure?

I want the properties of exhaust gases like viscosity, thermal conductivity, pradantal number at 4 bar pressure. Heat Mass data book mostly gives these values at 1 bar pressure.

06 February 2019 1,440 6 View

Medication Adherence Scale ?

As MMAS-8 is copyrighted, and the authors does not give permision to use it , is there any validated Medication Adherence Scale/ tool which is free to use in research or academic purposes?

11 September 2018 174 3 View

Does cooling process affect the morphology of carbonized material in pyrolysis?

We prepare carbon nanospheres from agricultural waste by pyrolysis. I have two questions: 1. Is there any evidence that explains formation of spherical shape during pyrolysis? 2. After pyrolysis,...

27 May 2018 4,960 3 View

Is there any method to quantify dispersion?

The carbon nanomaterial, we synthesized, is insoluble in most of the solvents. It forms suspension when dissolved. How can I quantify dispersion or how to determine the degree of dispersion in...

09 May 2018 6,399 7 View

I am presently working on DROUGHT TOLERANT MECHANISMS IN COTTON. What kind transcriptomics of work I should do in cotton?

I am working on Cotton drought tolerance. i am now doing the proteomics and metabolomics related things. What kind of new things I can do further that means any kind of transcriptomics or other...

04 February 2018 9,645 3 View

Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence?

I am on the lookout for the Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence. Does anyone know where I can find it? Thank you in advance

03 March 2021 3,568 1 View

Does anyone know HRM channel in a 2 plex QIAGEN Rotor- Gene Q qPCR can be used as a separate channel?

I am going to have 3 different probes in my qPCR work that I am going to do. But I realized that the machine we have in the lab is a Rotor-Gene Q 2plex HRM Platform, saying it has green, yellow,...

01 March 2021 8,544 1 View

Which cell line should we use to perform biological dosimetry experiments?

We are preparing some experiments based on irradiating cells under different conditions in order to evaluate the effects in terms of DNA damage, genetic expression, etc. As our project is...

01 March 2021 3,355 3 View

Is CD44 degraded in lysosome together with hyaluronan after CD44 mediated endocytosis of hyaluronic acid?

Also when RHAMM binds hyaluronic acid, they get internalized, will RHAMM also be degraded? Or both CD44 and RHAMM will be transferred back to the cell membrane? Asking for breast cancer cell line...

01 March 2021 8,169 2 View

LEDs on Vis Light photo degradation of MB water sol via N doped TiO2 in a mini flume?

Good day to all, I have no experience with light emitting diodes. I am building a glass mini flume to demonstrate the photo degradation of Methylene Blue in water running over self cleaning...

26 February 2021 4,026 5 View

Why do a weight decrease for thermal degradation in nitrogen gas condition?

Hi all The question is related to TGA(Thermogravimetric analysis). Thermal degradation easily understood but why do samples weight decrease? altough just binding is broked, the weight of...

26 February 2021 1,100 5 View

Calculating volume of DNA required from DNA concentration?

Hi I am a bit confused. They are asking me to find out the volume of DNA required in ul (a total of 30-100 ng for genomic DNA) from the DNA concentration in the nanodrop reading which was 404.8...

26 February 2021 5,029 2 View

Has anyone used the Illumina ITS1 primer sets?

Hi everyone, Illumina provides a list of primers to amplify with high taxonomic coverage the ITS1 region for further fungal sequencing, but I cannot find the exact amount necessary of each...

25 February 2021 6,969 3 View

Errors in DNA sequences?

I am trying to better understand the scope of DNA replication and sequencing errors, e.g. 1. I have seen similar error rates of 10e4 to 10e5 for cell & instrumental DNA replication,...

24 February 2021 4,397 3 View

Steps to calculate activation energy by coats & redfern method?

I'm a chemistry student and I'm researching the kinetic degradation of mixed plastic waste. I'm having trouble calculating the activation energy by the coats & Redfern method. The data that...

24 February 2021 974 1 View