Supriya Kammar

9 Questions 7 Answers 0 Followers

Questions related from Supriya Kammar

How to check whether the qPCR primers degraded or not?

I had transformed my desired gene along with selectable marker into rice plants and started with gene expression studies. Previously Amplification was observed in both genes but now on repetition,...

15 March 2024 7,800 1 View

Which software is the best for studying root architecture in plants?

Hi, Is there any free application I can use to study root morphology? The following information would be helpful to me: primary and lateral root lengths, root density, and root area. Thank you

12 January 2024 6,380 2 View

Which are the small factors that leads to DNA degradation by manual method?

Hello, I am working on rice transformation. I want to do restriction digestion of whole rice genome. For which I am isolating rice genome by manual CTAB method. But I am facing problem in every...

25 November 2022 3,893 2 View

Does rice root total protein degrade on heat treatment?

Hi researchers I am working specifically on rice root proteins for proteomics. Now I am at the stage of standardization of extraction buffer for rice roots. I followed Raorane, Narciso, and Kohli...

21 March 2022 7,546 0 View

PCR negative for transgenic plants?

Hi, I have obtained putative transgenic Nipponbare plants by 3 successive selection on antibiotic media. But I am getting negative results for PCR whereas, I got amplification in housekeeping gene...

09 November 2021 1,966 3 View

Can anyone suggest what kind of beads can be used in bead homogenizer for homogenizing plant tissue sample?

I have used glass beads for homogenizer but its forming powder. I want to know which beads are good to use for homogenizer. Thank you very much

30 July 2021 5,482 2 View

Can anyone suggest me good protein extraction protocol from rice root sample?

I was looking for protein extraction protocol from rice root which can provide me good result and taking into consideration that these extracts will be further analyzed with Orbitrap Mass...

01 June 2021 1,565 5 View

Can we detect and quantify 6-BAP or BA in Rice plants?

We submitted a sample of rice root for the purpose of quantifying phytohormones. Upon receiving the results, it was found that the sample contained BAP, a synthesized phytohormone that is...

01 January 1970 2,146 2 View

Positive qPCR for GOI initially but negative qPCR for GOI with same annealing temperature on repetition.

Hello researchers, For now, I have developed PCR-confirmed and antibiotic-selected stable transgenic rice plants. I was doing qPCR expression analysis for my gene of interest, but I am getting no...

01 January 1970 6,812 1 View