What to do with absence of fluorescence label in Fucci2a mouse-derived fibroblast culture?

Dear all, I have harvested fibroblast cells from R26p-Fucci2 mouse embryos (mouse line as described in this paper: https://dev.biologists.org/content/140/1/237) and cultured them on plastic,...

17 February 2021 3,368 2 View

(Scale request) Anyone has the Malay version of Eating Attitude Test-26/40 (EAT-26/40)?

Dear Researchers, My research team is conducting a eating disorder related research in Malaysia and we would like to use the Malay translated version of EAT26/40. I have tried to email the...

24 January 2021 8,925 2 View

Difficult to silence gene?

I have been trying to silence a gene of interest but I could not get silencing efficiencies beyond 50% My method of detection is via RT-qPCR and samples were extracted 48hrs post transfection. Are...

09 January 2021 3,404 4 View

There are prominent crystal formation in my cell culture dish, what are the possible causes and how to get rid of them?

17 December 2020 3,093 4 View

Using multiple linear regression to solve a multiple-way ANOVA problem?

Specifically, I want to know if I can use multiple linear regression to predict a response value 'y' from it's associated "treatment" or "group" averages in several variables. Let's say:...

04 November 2020 632 19 View

How to use a .pdb file as structure template to modeling on Chimera 1.14 ?

Hi, I need to use an own file as a template to modeling on Chimera (using Modeller). When I try to blast my sequence only find the option to query structures available on PDB Web Site. Does...

26 October 2020 2,436 3 View

What corporate social responsibilities do social media companies have in relation to the use of psychographic profiling in advertising ?

What are the responsibilities of CSR ? What social media companies have done to protect the privacy or information of their users ?

12 October 2020 433 5 View

Can you share your online teaching-learning experience during this pandemic period?

I have started online classes for undergrad, master, and Ph.D. students last January 16 up to the end of May. I have request students to allow recording classes and to discuss the process at the...

14 September 2020 4,466 23 View

May I know is Triple Negative cell line MDA-MB-468 is auto fluorescence ?

I am doing immunofluorescence using MDA-MB-468 cell to determine ICAM-1 and E-cadherin with Santa Cruz Biotechnology ICAM-1 (G-5) mouse anti-human (Primary antibody), Santa Cruz Biotechnology...

27 August 2020 1,641 4 View

Can anti-Fas (anti-CD95) be used to prepare apoptotic cancer cell as antigens for DC to present antigen?

Researchers usually used irradiation (such as x-ray) to induce cancer cell apoptosis to prepare available antigens for DC to uptake that present the antigen to T cells. Can other apoptotic methods...

26 August 2020 5,445 4 View

Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence?

I am on the lookout for the Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence. Does anyone know where I can find it? Thank you in advance

03 March 2021 3,568 1 View

Does anyone know HRM channel in a 2 plex QIAGEN Rotor- Gene Q qPCR can be used as a separate channel?

I am going to have 3 different probes in my qPCR work that I am going to do. But I realized that the machine we have in the lab is a Rotor-Gene Q 2plex HRM Platform, saying it has green, yellow,...

01 March 2021 8,544 1 View

Which cell line should we use to perform biological dosimetry experiments?

We are preparing some experiments based on irradiating cells under different conditions in order to evaluate the effects in terms of DNA damage, genetic expression, etc. As our project is...

01 March 2021 3,355 3 View

Calculating volume of DNA required from DNA concentration?

Hi I am a bit confused. They are asking me to find out the volume of DNA required in ul (a total of 30-100 ng for genomic DNA) from the DNA concentration in the nanodrop reading which was 404.8...

26 February 2021 5,029 2 View

Has anyone used the Illumina ITS1 primer sets?

Hi everyone, Illumina provides a list of primers to amplify with high taxonomic coverage the ITS1 region for further fungal sequencing, but I cannot find the exact amount necessary of each...

25 February 2021 6,969 3 View

Errors in DNA sequences?

I am trying to better understand the scope of DNA replication and sequencing errors, e.g. 1. I have seen similar error rates of 10e4 to 10e5 for cell & instrumental DNA replication,...

24 February 2021 4,397 3 View

What might be the reason for obtaining two bands in plant genomic DNA extraction?

I got a thin band and a thick band i guess it would be the genomic DNA and the 260/280 ratio is 3.

21 February 2021 6,523 6 View

I can detect my protein but the encoding DNA sequence has stop codons within. Should I ignore the stop codons?

I constructed a synthetic DNA library (scFv, VH-VL orientation) with a 3' reporter and 8x histag. I cloned and expressed this gene following which I performed an ELISA. The ELISA results suggested...

19 February 2021 7,006 5 View

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17 February 2021 4,217 3 View

How much amount of DNA (ligation mix) should I use for transformation?

Hi Fellow Scientists! I am trying to clone my 300 bp insert into a 6 kb vector. I run a few different ligation reactions using (1:3 vector: insert molar ratios) 10, 20,30, and 40 ng/ul vector...

15 February 2021 9,563 6 View