Is there a limit on the protein size?
Which parameters should I have in mind to choose one or the other?
I am most familiar with using text boxes in WORD but I wasn't sure if that would be acceptable.
11 January 2021 3,585 1 View
If possible any easy preparation method
31 December 2020 1,662 3 View
Hi all, this is my first-time culturing lymphoma cell line. I obtained a frozen vial of EL4 (in the background of B6 mice) lymphoma cell line, and I am the only one culturing this line in the...
04 November 2020 932 3 View
I am organizing a conference for the first time and I would like to partner with an University for the conference. What are the things that I should do to invite/request a university. Whom should...
04 October 2020 7,530 3 View
Hello everyone. I am an undergraduate student majoring in cardio-thoracic technology. I'm now working on my Bachelor's thesis that i use real time PCR (gene expression study between drug-treated...
11 August 2020 4,594 9 View
We have conducted an experiment on epigenetic allele identification and quantification for some quantitative trait. we are trying to map epigenes using epigenome association mapping (EWAS)....
10 August 2020 4,170 3 View
Advances in data science throwing its impact on agriculture research data analysis. looking to understand applications of machine learning concepts in agriculture research. Thanks
29 June 2020 6,959 6 View
Hi, I found a number of behavioural papers studying anxiety behaviour following stress. Stress was induced by exposure to predators, agonistic interaction or electric shocks. But these are almost...
24 June 2020 309 1 View
How can a student be able to find open access journals with no publication fees to publish their work? A step by step procedure will be most appreciated.
04 May 2020 6,700 3 View
Is there any correlation between the impact factor of scientific journals and the average rate and time for rejection. Many high impact journals reject submissions even before peer review.
04 May 2020 262 14 View
I am on the lookout for the Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence. Does anyone know where I can find it? Thank you in advance
03 March 2021 3,568 1 View
I have a set of stably transfected cell lines all transfected with plasmids containing GFP tags on the C terminus. During a western blot using anti-GFP antibody, one of my plasmids has dissociated...
01 March 2021 9,310 4 View
Hi, I am running a size exclusion chromatography experiment with a buffer containing Potassium Acetate as a salt. I analyse these fractions through SDS-PAGE. After boiling my SEC fractions in...
01 March 2021 2,622 3 View
I have used the i-Tasser several times, however it has been unavailable for several days. I tried the swiss-model, but the output was not very pleasant due to the model used. Is there any other...
28 February 2021 4,521 3 View
I'm a student and I have to produce a cell line with a knockin for the NRF2 gene with GFP. I have to put a promotor in front of the GFP because the gene will be too far away from the promotor of...
28 February 2021 7,127 2 View
I have used an AllPrep DNA/RNA/Protein Mini Kit QIAGEN kit to extract RNA and protein from my samples. At the end of the protein purification, I resuspend my protein in 5% SDS. Will these samples...
28 February 2021 7,370 3 View
I had a crystal hit in a well with 0.1M HEPEs pH 7.5, 10%(w/v) PEG 4000, and 20%(w/v) isopropanol. Why is isopropanol in w/v here if it's just solvent? How do I recreate this well condition?
25 February 2021 9,761 1 View
I'm currently working having problem with the concentration for recombinant protein use in western blot, the one been used is Recombinant Human CD19 Fc Chimera Protein-rndsystem which does not...
24 February 2021 8,514 3 View
I am trying to clone 2 copies of the same mammalian gene into the pSF-CMV-Ub-Puro Ascl (contains HindIII, KpnI and NheI cut sites) plasmid. This is what the final product is supposed to look like...
24 February 2021 6,310 3 View
Hi all, I have been doing research on biotagged LDB1 proteins in Mel cells. I purify the proteins using M280 streptavidin beads. The yield is very low so I've been trying to optimize it by...
24 February 2021 5,749 3 View