When reporting mediation in a manuscript being submitted for publication, what have you found to be the easiest way to create your path diagram?

I am most familiar with using text boxes in WORD but I wasn't sure if that would be acceptable.

11 January 2021 3,585 1 View

What method is more useful for prepare metal/ metal oxide Nanocomposites?

If possible any easy preparation method

31 December 2020 1,662 3 View

Advice on the culturing of EL4 lymphoma cell line?

Hi all, this is my first-time culturing lymphoma cell line. I obtained a frozen vial of EL4 (in the background of B6 mice) lymphoma cell line, and I am the only one culturing this line in the...

04 November 2020 932 3 View

Is it possible that a protein band appears on a western blot but not detected on ELISA?

I am working on recombinant protein expression in E. coli. Recently, I thought I had expressed a certain protein successfully as I could see a band of the expected size - 17 KDa (this band was...

14 October 2020 5,144 4 View

How to find a partner university for an Academic conference?

I am organizing a conference for the first time and I would like to partner with an University for the conference. What are the things that I should do to invite/request a university. Whom should...

04 October 2020 7,530 3 View

How does RNA mini kit get rid of DNA without using additional DNase treatment?

Hello everyone. I am an undergraduate student majoring in cardio-thoracic technology. I'm now working on my Bachelor's thesis that i use real time PCR (gene expression study between drug-treated...

11 August 2020 4,594 9 View

Can any one help me to perform EWAS ?

We have conducted an experiment on epigenetic allele identification and quantification for some quantitative trait. we are trying to map epigenes using epigenome association mapping (EWAS)....

10 August 2020 4,170 3 View

Is it possible to fix a co-culture of bacteria and human cells in 96 well plates and use the plates at a later point for flow cytometry analysis?

I am co-culturing bacterial strains with human cells (WBCs) and there are about 3000 different bacterial strains. The idea is to carry out flow cytometry experiments with these samples (in 96 well...

09 July 2020 3,106 5 View

Application of machine learning in agriculture research?

Advances in data science throwing its impact on agriculture research data analysis. looking to understand applications of machine learning concepts in agriculture research. Thanks

29 June 2020 6,959 6 View

Behaviour, inducing stress in terrestrial invertebrates?

Hi, I found a number of behavioural papers studying anxiety behaviour following stress. Stress was induced by exposure to predators, agonistic interaction or electric shocks. But these are almost...

24 June 2020 309 1 View

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02 March 2021 3,060 3 View

Issues with Permeabilization during Flow Cytometry?

Hello Everyone. Currently I am working to characterize macrophages in the myocardium after ischemia-reperfusion injury in rats. Due to the low total cell number isolated from rat hearts I can...

01 March 2021 3,867 3 View

Which cell line should we use to perform biological dosimetry experiments?

We are preparing some experiments based on irradiating cells under different conditions in order to evaluate the effects in terms of DNA damage, genetic expression, etc. As our project is...

01 March 2021 3,355 3 View

Is it possible for two human neuronal cell lines to have different gene sequence for the same protein?

I am looking at the ATP1A2 (Sodium/Potassium ATPase alpha subunit 2) in two human neuronal cell lines. Expression levels of this protein seems to be almost equal when detected by one antibody....

01 March 2021 3,607 3 View

Is CD44 degraded in lysosome together with hyaluronan after CD44 mediated endocytosis of hyaluronic acid?

Also when RHAMM binds hyaluronic acid, they get internalized, will RHAMM also be degraded? Or both CD44 and RHAMM will be transferred back to the cell membrane? Asking for breast cancer cell line...

01 March 2021 8,169 2 View

Do you need the CMV enhancer before the CMV promotor for a proper function of CMV on the gene that follows?

I'm a student and I have to produce a cell line with a knockin for the NRF2 gene with GFP. I have to put a promotor in front of the GFP because the gene will be too far away from the promotor of...

28 February 2021 7,127 2 View

Interested to stain mice tissue with WGA 488 conjugate, anybody has a protocol to recommend?

Interested to stain mice brain tissue with WGA 488 conjugate, appreciate if anyone willing to offer me some guidance or suggestions! Thank you!

28 February 2021 3,951 2 View

What could be some reasons why unstimulated monocytes are getting activated?

Im doing PBMC isolation -> CD14+ enrichment using magnetic beads -> stimulation setup. My negative control is just cells in cRPMI but they seem to get activated over and over again.

28 February 2021 7,883 3 View

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28 February 2021 9,936 2 View

B-mercaptoethanol (BME) protocol?

Hi everybody, I have been running a multiplex IHC which allow me to stain the same tissue with different markers (Primary Ab + Secondary conjugated Ab) (AF487, AF555, AF647). Since I would like to...

27 February 2021 5,028 2 View