In order to view/ find the laid thrip eggs is there any methods is available, if yes, suggest me the methods.
Mangroves were reported to eat into limestone atolls like so much cake - but could it have been diatom partnerships at mangrove roots that ate into the rock? If gypsum formation is a clue, how...
12 July 2024 159 2 View
During the release test of Amphotericin B using 1% Tween 80, i observed three peaks even in the standard. The first peak consistently yielded results around 500%, while the other peaks showed...
08 July 2024 1,330 0 View
Hello, I have been performing Western Blots to study the protein levels of a rodent skin and muscle sample. However, I have not been able to produce homogenous reference bands. I suspect that...
26 June 2024 5,913 3 View
When I analyzed grades of undergraduates, some course taught via online platforms, their grades are lower than the physical lectures.
11 May 2024 7,475 4 View
Agroforestry is considered and identified as a good land management practice. But most of the farmers prefer monoculture or mixed cropping system, But not agroforestry provide lots of tangible and...
09 May 2024 2,376 2 View
Hello, I hope this message finds you well. I have just started to learn gaussian software. While running NLO calculations I used the keyword: #p b3lyp/6-31g(d,p) geom=connectivity gfinput...
08 May 2024 6,756 2 View
The inclusion of deformation energy and entropy corrections in MMPBSA binding free energy calculations is common in studies involving DNA-ligand complexes. However, in the literature, these terms...
25 April 2024 7,286 0 View
The Marquis de Lafayette is "returning" to America again this fall. After serving in the American and French Revolutions, he returned to America in 1824-1825 and toured some 25 states. This...
19 April 2024 4,979 0 View
I have got measurement results from China. But they have given me the results in tfh file format. Can anyone suggest a software to open the file and analyse the results?
14 April 2024 789 3 View
Hello! I am trying to characterize communities of eukaryotes living in biofilms using V4 region 18s amplicon sequencing data. Working with this type of data is new to me, and very interesting!...
04 April 2024 6,603 1 View
I have carried out MFC experiments on three different volumes, 50, 500 and 1000 mL of wastewater. Results after MFC treatment shows that TDS and EC are more in larger volumes of water i.e. TDS and...
09 August 2024 9,621 0 View
Hello everyone! I observed in my culture (htert-RPE1 cells) an orange- red particle at the bottom of the dish. It is visible to the naked eye as a very very small red dot. Could it be a...
09 August 2024 2,824 3 View
I am working in fungal fermentation of soybean meal and there is bacterial growth in them at times. I am trying to quantify fungal cell counts and bacterial cells; but I haven't been able to do at...
07 August 2024 7,535 4 View
I am staining some brain sections stored in cryoprotectant that express a Histone H2B- GFP fusion protein that were generated ~10 years ago. I know I need to enhance signal with an anti-GFP...
07 August 2024 5,338 2 View
Hi, I have a question about normalizing the MTT OD values for doing the statistical analysis. So, if we have 3 different plates and we call them 3 different replicates, so, first we would...
07 August 2024 8,106 4 View
Previously when I co-coluture anti-CD19(FMC63) CAR-Jurkat with Raji with E:T=5:1, Jurkat can eliminate Raji in 24h. However, when I test another CAR construct, although I can dectect totally CD69...
06 August 2024 641 2 View
To compare positive and negative cell populations in flow cytometry, should I compare unstained cells with antibody stained cells? Or with the isotype control? Most papers show comparison with...
06 August 2024 6,728 6 View
Hi all, I was just wondering if anyone has experience with multiplexing a mouse monoclonal primary and a rat primary. I'm trying to multiplex by incubating them in the same well but was told by a...
06 August 2024 9,710 1 View
I have tried several times to isolate lymphocytes from mouse spleen, but all attempts have been unsuccessful. I tried most available protocols. I used different dissociation media (HBSS with Ca...
04 August 2024 9,913 7 View
I have protein-membrane simulations (PDB, PSF, DCD) and have noticed that water molecules near the protein are not visible in the simulations. How can I fix this issue? Is there a way to place the...
04 August 2024 1,200 2 View