Why Pseudomonas fluorescens ATCC13525 does not grow on acetate?

I am trying to grow Pseudomonas fluorescens ATCC13525 on different carbon source except glucose in M9 medium but i cannot see them growing on acetate. I am not able to find more information about...

01 February 2020 7,130 4 View

Is raman peak at 515cm-1 normal for silicon wafer with 532 laser and 600 grating?

We installed new grating to your Renishaw inVia Raman microsscope. While routine calibration of the system, we observed the Si raman peak at 515cm-1 for 532 lase 600 grating and 519.9 cm-1 for 532...

11 December 2019 5,845 3 View

What are some of the best substrate that we can use for Raman measurement of bacteria except CaF2?

I am looking for the cheaper substrate for Raman measurement of bacteria, without compromising much on the quality of spectra, also does anybody have experience with metal coated slide for...

05 June 2019 6,310 3 View

How to calculate signal to noise ratio ?

I have raman spectrum from a single bacterial cell, i want to calculate the signal to noise ratio for the same, what is the correct way of doing it?

01 February 2019 2,134 4 View

What should be the ideal size of Au or Ag nanoparticles for getting optimum SERS effect ?

I want to perform SERS for bacteria, i want to know what should be the ideal size of Au or Ag nano-particles which can create optimum plasmonic effect considering also the stability of the particles.

09 October 2017 3,982 4 View

What is indicated by Raman intensity for bacteria?

01 February 2017 8,245 3 View

Can we use glassy carbon as the substrate for Raman Microscopy?

Want to study the behavior of bacteria on glassy carbon, so i wanted to know if i can directly use glassy carbon slide as substrate for Raman microbial analysis??

31 December 2016 9,246 1 View

What is Convective assembly method for SERS?

Recently i was reading an article on SERS for bacterial detection, there i came across this work "Convective assembly method" for increasing the reproducibility of SERS signal. I googled it, but...

05 June 2016 5,943 3 View

How to measure the Zeta potential of Bacteria?

Hello friends, I tried to measure zeta potential of bacteria in 10 mM PBS by malvern zetasizer, but during measurement both the electrodes turned black and my bacterial culture precipitated and...

01 February 2016 7,035 7 View

what is the simplest definition of ion exchange capacity

What this unit identifies for the ion exchange capacity ca. 0.92 meq. g-1. I am a newcomer in the membrane area. I just started. I highly appreciate your help

08 May 2021 0 0 View

what is the simplest definition of ion exchange capacity

What this unit identifies for the ion exchange capacity ca. 0.92 meq. g-1. I am a newcomer in the membrane area. I just started. I highly appreciate your help

08 May 2021 0 0 View

Is There Any Feasible Method To Test Fluorescent Compounds Other Than Fluorescence Spectrometers ??

Is There Any Feasible Method To Test The Efficiency Of Fluorescent Compounds Other Than UV Spectrometers ? Suggestions Would Be Appreciated !

02 March 2021 5,785 3 View

Does anyone know HRM channel in a 2 plex QIAGEN Rotor- Gene Q qPCR can be used as a separate channel?

I am going to have 3 different probes in my qPCR work that I am going to do. But I realized that the machine we have in the lab is a Rotor-Gene Q 2plex HRM Platform, saying it has green, yellow,...

01 March 2021 8,544 1 View

Samples to study circular dichroism (chirality)?

I want to build a low-cost polarimeter to study circular dichroism (chirality). What type of samples can I use? I am looking for samples that can be found outside the laboratory.

01 March 2021 9,101 3 View

What are the new 2D nanomaterials were used for Silver nanoparticles stabilization?

In the stabilization of AgNPs are vital in current research interest. However, some of the 2D nano-materials like graphene oxide, MoS2, etc... previously used to stabilize it. But still they shown...

01 March 2021 2,207 3 View

How to accurately quantify chitin?

Hi all, I have been working on the quantification of chitin in insect samples and feed for a while but I can notice that the quantification methods (e.g. exposure to HCl, NaOH, and weighing sample...

01 March 2021 1,843 2 View

Dapi fluorescence protocol for colon cell lines ?

28 February 2021 2,534 2 View

How does charge on working electrode changes with rising potential?

During performing CV of a GCE or modified GCE, we rise the potential in the first half of the cycle and reduce it in reverse half. During the first half, what is the nature of charge on GCE or...

25 February 2021 7,994 3 View

Can anyone help with Schistosoma identification?

Can anyone identify what kind of Schistosome species this might be? Morphological features of cercariae and adults resemble Trichobilharzia, though I am fairly new to the field of parasitology...

25 February 2021 7,760 6 View