Why Pseudomonas fluorescens ATCC13525 does not grow on acetate?

I am trying to grow Pseudomonas fluorescens ATCC13525 on different carbon source except glucose in M9 medium but i cannot see them growing on acetate. I am not able to find more information about...

01 February 2020 7,130 4 View

Is raman peak at 515cm-1 normal for silicon wafer with 532 laser and 600 grating?

We installed new grating to your Renishaw inVia Raman microsscope. While routine calibration of the system, we observed the Si raman peak at 515cm-1 for 532 lase 600 grating and 519.9 cm-1 for 532...

11 December 2019 5,845 3 View

How to calculate signal to noise ratio ?

I have raman spectrum from a single bacterial cell, i want to calculate the signal to noise ratio for the same, what is the correct way of doing it?

01 February 2019 2,134 4 View

What should be the ideal size of Au or Ag nanoparticles for getting optimum SERS effect ?

I want to perform SERS for bacteria, i want to know what should be the ideal size of Au or Ag nano-particles which can create optimum plasmonic effect considering also the stability of the particles.

09 October 2017 3,982 4 View

What is indicated by Raman intensity for bacteria?

01 February 2017 8,245 3 View

Which are some of the companies providing graphite membrane?

I want to use graphite membrane for the biofermenter to study diffusion of ions. But i cannot find any company offering good ranges of graphite membrane. Answers are requested from somebody...

31 December 2016 9,137 2 View

Can we use glassy carbon as the substrate for Raman Microscopy?

Want to study the behavior of bacteria on glassy carbon, so i wanted to know if i can directly use glassy carbon slide as substrate for Raman microbial analysis??

31 December 2016 9,246 1 View

What is Convective assembly method for SERS?

Recently i was reading an article on SERS for bacterial detection, there i came across this work "Convective assembly method" for increasing the reproducibility of SERS signal. I googled it, but...

05 June 2016 5,943 3 View

How to measure the Zeta potential of Bacteria?

Hello friends, I tried to measure zeta potential of bacteria in 10 mM PBS by malvern zetasizer, but during measurement both the electrodes turned black and my bacterial culture precipitated and...

01 February 2016 7,035 7 View

I need to image mesoporous silica nanoparticles that are in ~100 nm size and do high resolution TEM to look at mesopores. what grids should I use?

Need to image mesoporous silica nanoparticles using the TEM. Also, need high resolution TEM images to see the mesoporous structure. Kindly suggest what kind of grids to use. Thanks, Shatadru

02 March 2021 1,787 2 View

Is There Any Feasible Method To Test Fluorescent Compounds Other Than Fluorescence Spectrometers ??

Is There Any Feasible Method To Test The Efficiency Of Fluorescent Compounds Other Than UV Spectrometers ? Suggestions Would Be Appreciated !

02 March 2021 5,785 3 View

Shall I skip the measurement model in SmartPLS?

Hello everyone, I had run a mediation model in SmartPLS using questionnaires that were already validated among my statistical population. A professor who edited my paper -though she is not...

01 March 2021 3,052 3 View

Adherance of Polyaniline coating over stainless steel?

Is the electropolymerisation of polyaniline coating over stainless steel (SS) via Cyclic voltammetry technique is really adherant? Else how to improve the adhesion of the coating, whether any...

01 March 2021 8,220 2 View

Is there a method of determining feed digestibility without the need to re-pellet the feed?

I am aiming to determine the ADC values of a commercial fish feed, but I do not have a steam pelleter or extruder available to me. Ideally, the pellets could be coated with some inert marker....

28 February 2021 7,967 3 View

How to immerse bricks in wax ( for coating ) ?

Do we immerse the bricks horizontally or vertically ? #bricks#waterproofingsurface#civil#engeenering#chemicalengineering#wax#parafinwax

28 February 2021 6,685 2 View

For gelatin coating for culturing HUVEC.Should the gelatin be made in sterile water or PBS? Do I need to autoclave the gelatin soln after making it ?

I want to use 0.1% gelatin. Should that be made in PBS or sterile water? Should I autoclave the gelatin soln after making it in water/PBS?

28 February 2021 274 2 View

Why is my platinum counter electrode getting dirty during electrodeposition?

I am conducting anodic electrodeposition of MnO2 onto a stainless steel substrate, using a platinum strip as a counter electrode. This is done galvanostatically, with the current held between 5...

27 February 2021 1,829 3 View

SEM or Multiple Regression for an analysis without Latent Variables?

Hello, I have three hypothesis to test, all with the same IV. I am testing if the effect of the IV on the first DV is mediated by awe. I am then measuring whether the effect of the IV on a...

26 February 2021 8,399 5 View

What pH condition required to coat APTES on silica coated Fe3O4?

It's a hydrolysis reaction, which may produce by either acid or base-catalyzed reaction. so can anybody help me with this, which condition is suitable for this reaction?

26 February 2021 1,760 8 View