Want to study the behavior of bacteria on glassy carbon, so i wanted to know if i can directly use glassy carbon slide as substrate for Raman microbial analysis??
Thanks
I am trying to grow Pseudomonas fluorescens ATCC13525 on different carbon source except glucose in M9 medium but i cannot see them growing on acetate. I am not able to find more information about...
01 February 2020 7,266 4 View
We installed new grating to your Renishaw inVia Raman microsscope. While routine calibration of the system, we observed the Si raman peak at 515cm-1 for 532 lase 600 grating and 519.9 cm-1 for 532...
11 December 2019 5,984 3 View
I am looking for the cheaper substrate for Raman measurement of bacteria, without compromising much on the quality of spectra, also does anybody have experience with metal coated slide for...
05 June 2019 6,398 3 View
I have raman spectrum from a single bacterial cell, i want to calculate the signal to noise ratio for the same, what is the correct way of doing it?
01 February 2019 2,241 4 View
I want to perform SERS for bacteria, i want to know what should be the ideal size of Au or Ag nano-particles which can create optimum plasmonic effect considering also the stability of the particles.
09 October 2017 4,069 4 View
I supplemented bacteria with 13C labelled substrate, and measured the incorporation of labelled growth substrate at regular time interval with Raman Microscope. Incorporation of labelled substrate...
01 February 2017 8,325 3 View
I want to use graphite membrane for the biofermenter to study diffusion of ions. But i cannot find any company offering good ranges of graphite membrane. Answers are requested from somebody...
31 December 2016 9,220 2 View
Recently i was reading an article on SERS for bacterial detection, there i came across this work "Convective assembly method" for increasing the reproducibility of SERS signal. I googled it, but...
05 June 2016 6,033 3 View
Hello friends, I tried to measure zeta potential of bacteria in 10 mM PBS by malvern zetasizer, but during measurement both the electrodes turned black and my bacterial culture precipitated and...
01 February 2016 7,131 7 View
I have virus (viral hemorrhagic septicemia virus) in suspension and the experiment will not involve cells. What level of TCID50 is preferred?
11 August 2024 3,115 1 View
I tried four trials of the same Copper Phosphides sample in Alkaline medium ( 0.5M KOH) with Hg/HgO reference electrode and Pt as counter electrode. I used 0.001 V/s scan rate for first three...
10 August 2024 3,629 1 View
I would like to understand potential safety concerns while handling SEB in the lab. Especially while working in animal house facility. Would like to know precautions for handling. Sigma MSDS...
07 August 2024 6,034 3 View
Hello dear colleagues, We have prepared a manuscript on NiTi-based alloys and are seeking a second opinion on our current TEM results. If you are a Ph.D. holder with experience in TEM and have...
07 August 2024 9,563 0 View
After immunohistochemistry of previously fixed in PFA and EtOH and then frozen 20 μm sections of zebrafish brain, DAPI staining is very weak (right) compared to the same sections stained without...
05 August 2024 9,637 2 View
There are anaerobic gas pak jars and environment providers in the laboratory that provide anaerobic environmental conditions for the growth of anaerobic bacteria. However, there is no anaerobic...
05 August 2024 4,945 3 View
average particle size calculation from TEM
04 August 2024 2,921 1 View
I have been using paraffin, but the deposited ZnO still detach from electrode. What is the best binder to modify graphite paste electrode with ZnO nanoparticles?
03 August 2024 4,624 3 View
I am working on a SEM model where i have 7 latent variables (6 exogenous and 1 endogenous). In AMOS when I co-vary the exogenous constructs, only 2 paths are coming significant out of 6. But when...
03 August 2024 6,028 4 View
Suppose I am studying the construct of "Academic Motivation" (latent variable) in students, which is measured through three observed variables: "Intrinsic Motivation," "Extrinsic Motivation," and...
31 July 2024 3,913 6 View