Why Pseudomonas fluorescens ATCC13525 does not grow on acetate?

I am trying to grow Pseudomonas fluorescens ATCC13525 on different carbon source except glucose in M9 medium but i cannot see them growing on acetate. I am not able to find more information about...

01 February 2020 7,130 4 View

Is raman peak at 515cm-1 normal for silicon wafer with 532 laser and 600 grating?

We installed new grating to your Renishaw inVia Raman microsscope. While routine calibration of the system, we observed the Si raman peak at 515cm-1 for 532 lase 600 grating and 519.9 cm-1 for 532...

11 December 2019 5,845 3 View

What are some of the best substrate that we can use for Raman measurement of bacteria except CaF2?

I am looking for the cheaper substrate for Raman measurement of bacteria, without compromising much on the quality of spectra, also does anybody have experience with metal coated slide for...

05 June 2019 6,310 3 View

How to calculate signal to noise ratio ?

I have raman spectrum from a single bacterial cell, i want to calculate the signal to noise ratio for the same, what is the correct way of doing it?

01 February 2019 2,134 4 View

What should be the ideal size of Au or Ag nanoparticles for getting optimum SERS effect ?

I want to perform SERS for bacteria, i want to know what should be the ideal size of Au or Ag nano-particles which can create optimum plasmonic effect considering also the stability of the particles.

09 October 2017 3,982 4 View

What is indicated by Raman intensity for bacteria?

01 February 2017 8,245 3 View

Which are some of the companies providing graphite membrane?

I want to use graphite membrane for the biofermenter to study diffusion of ions. But i cannot find any company offering good ranges of graphite membrane. Answers are requested from somebody...

31 December 2016 9,137 2 View

What is Convective assembly method for SERS?

Recently i was reading an article on SERS for bacterial detection, there i came across this work "Convective assembly method" for increasing the reproducibility of SERS signal. I googled it, but...

05 June 2016 5,943 3 View

How to measure the Zeta potential of Bacteria?

Hello friends, I tried to measure zeta potential of bacteria in 10 mM PBS by malvern zetasizer, but during measurement both the electrodes turned black and my bacterial culture precipitated and...

01 February 2016 7,035 7 View

I need to image mesoporous silica nanoparticles that are in ~100 nm size and do high resolution TEM to look at mesopores. what grids should I use?

Need to image mesoporous silica nanoparticles using the TEM. Also, need high resolution TEM images to see the mesoporous structure. Kindly suggest what kind of grids to use. Thanks, Shatadru

02 March 2021 1,787 2 View

Is There Any Feasible Method To Test Fluorescent Compounds Other Than Fluorescence Spectrometers ??

Is There Any Feasible Method To Test The Efficiency Of Fluorescent Compounds Other Than UV Spectrometers ? Suggestions Would Be Appreciated !

02 March 2021 5,785 3 View

Sutter electrode holder leaking after applying positive pressure. How to fix?

I am using a Sutter electrode holder for my patch clamp experiments, but I'm having trouble keeping positive pressure inside my electrode due to a leak out of the back of the holder. I have added...

02 March 2021 5,829 1 View

What thickness need for ITO to use as conductive layer?

I want to do RF sputtering of ITO on glass substrate using magnetron sputtering. what thickness do i need to make it good conductive layer to use it for PSC. Moreover, whats about thickness of...

01 March 2021 395 3 View

Shall I skip the measurement model in SmartPLS?

Hello everyone, I had run a mediation model in SmartPLS using questionnaires that were already validated among my statistical population. A professor who edited my paper -though she is not...

01 March 2021 3,052 3 View

Why is my platinum counter electrode getting dirty during electrodeposition?

I am conducting anodic electrodeposition of MnO2 onto a stainless steel substrate, using a platinum strip as a counter electrode. This is done galvanostatically, with the current held between 5...

27 February 2021 1,829 3 View

What is the exact mechanism of the antimicrobial effect of local anesthetics?

The antimicrobial effect of local anesthetics has been known for more than 50 years, but it still seems to have an unclear mechanism of action. which bacteria? which concentration? etc etc. ....

27 February 2021 4,450 2 View

SEM or Multiple Regression for an analysis without Latent Variables?

Hello, I have three hypothesis to test, all with the same IV. I am testing if the effect of the IV on the first DV is mediated by awe. I am then measuring whether the effect of the IV on a...

26 February 2021 8,399 5 View

How does charge on working electrode changes with rising potential?

During performing CV of a GCE or modified GCE, we rise the potential in the first half of the cycle and reduce it in reverse half. During the first half, what is the nature of charge on GCE or...

25 February 2021 7,994 3 View

Can anyone help with Schistosoma identification?

Can anyone identify what kind of Schistosome species this might be? Morphological features of cercariae and adults resemble Trichobilharzia, though I am fairly new to the field of parasitology...

25 February 2021 7,760 6 View