Difficulty in debugging code to correct error - TypeError: '<' not supported ?

I am trying with a Unet code ontained from net ...in tensorflow. When trying to predict the segmentation result with the trained and saved model with metric " iou" using "CustomObjectScope"...

22 February 2021 5,254 1 View

Is there a specific dimension of Morris water maze to assess the spatial memory in MPTP induced Parkinson's disease model of C57 Bl6 mice?

I am trying to assess spatial memory in MPTP induced Parkinson's disease C57Bl6 mice using a Morris water maze. I wanted to know if there are any specific dimensions to the MWM to be used. Most...

17 November 2020 4,558 3 View

Give possible suggestions to include unorganised food processing industry into the industrial scenario?

In the food processing industry many of it are unorganised one. In that case we couldn't take into account for analysis. Those firms are not comes under registered unit.

24 January 2020 7,565 2 View

FT IR results revealed the presence of three marked and well defined peaks in the finger print region,does it have any significance?

ft ir analysis results

19 June 2019 2,245 7 View

Does Fe/ Fe oxide nanoparticle precipitate out of solution?

I was trying to synthesise Fe/Fe oxide naoparticle through green rout ( plant mediated). On adding and stirring the plant extract to precursor the solution changed colour to dark red and fine...

31 August 2018 4,457 6 View

Can anyone recommend me the type of grid for TEM measurement of Fe3O4 silica nanoparticles and only silica nanoparticles?

22 June 2017 1,008 3 View

Will any bacteria show fluorescence on Nutrient Agar medium?

We have recently isolated a strain, which is a gram variant strain showing mucoidal colony morphology on nutrient agar plates and which glows (luminescence/ fluorescence) when viewed under UV...

12 March 2017 1,472 3 View

How to calculate percentage drug release?

04 October 2016 3,083 12 View

Is it possible to calculate area measurement for change detected of Multitemporal images

Hi all, Can any one suggest me how to calculate change Area measurement in change detection of multitemporal satellite images.

31 August 2016 4,397 7 View

How to assess platelet count manually in peripheral smear with only clumps?

how to assess platelet count manually in a peripheral smear with only clumps?

27 May 2016 8,301 3 View

How can I measure MMP-1 protein activity or protein level from liver tissue?

Instead of checking only the mRNA level, I want to check the active protein level of MMP-1 in Liver tissue from mice. How can I do that?

03 March 2021 1,763 2 View

Can we classify HV and LV lines based on line length?

I have dataset which shows the length of power lines. I need to classify the lines based on the line length. Is there a rule to classify the High voltage (HV) and low voltage (LV) lines based on...

03 March 2021 4,116 4 View

Is There Any Feasible Method To Test Fluorescent Compounds Other Than Fluorescence Spectrometers ??

Is There Any Feasible Method To Test The Efficiency Of Fluorescent Compounds Other Than UV Spectrometers ? Suggestions Would Be Appreciated !

02 March 2021 5,785 3 View

IHC on mouse liver hepatocytes?

Can anyone help me choose primary antibodies for mouse liver hepatocytes and also in conjunction Cd8 cells. Also Cd3 cells. I want to look at hepatocytes and adjacent immune cells in mice with...

01 March 2021 6,953 1 View

Why might my GFP tag be dissociating from my plasmid after transfection?

I have a set of stably transfected cell lines all transfected with plasmids containing GFP tags on the C terminus. During a western blot using anti-GFP antibody, one of my plasmids has dissociated...

01 March 2021 9,310 4 View

Precipitate after adding laemmli buffer to protein solution containing Potassium Acetate?

Hi, I am running a size exclusion chromatography experiment with a buffer containing Potassium Acetate as a salt. I analyse these fractions through SDS-PAGE. After boiling my SEC fractions in...

01 March 2021 2,622 3 View

How to avoid ulcers in s.c. injected tumors ?

Recently, we have injected s.c. B16F10 tumor cells in BL/6 mice. At around 12-15th day some animals showed ulcerations in tumors , with a big hole in the middle (it looks like a volcano). We have...

01 March 2021 9,938 3 View

What is the most suitable server for predicting protein structures in bacteria?

I have used the i-Tasser several times, however it has been unavailable for several days. I tried the swiss-model, but the output was not very pleasant due to the model used. Is there any other...

28 February 2021 4,521 3 View

Do you need the CMV enhancer before the CMV promotor for a proper function of CMV on the gene that follows?

I'm a student and I have to produce a cell line with a knockin for the NRF2 gene with GFP. I have to put a promotor in front of the GFP because the gene will be too far away from the promotor of...

28 February 2021 7,127 2 View

Are protein samples in SDS compatible with ELISA?

I have used an AllPrep DNA/RNA/Protein Mini Kit QIAGEN kit to extract RNA and protein from my samples. At the end of the protein purification, I resuspend my protein in 5% SDS. Will these samples...

28 February 2021 7,370 3 View