Which Antibodies against AMPK and pAMPK do I use?

I'm trying to demonstrate that ghrelin activates AMPK in hippocampus and improves cognition in Parkinson's mice For the western blot which primary antibody should I use? For pAMPK...

21 January 2021 413 3 View

Is there a specific dimension of Morris water maze to assess the spatial memory in MPTP induced Parkinson's disease model of C57 Bl6 mice?

I am trying to assess spatial memory in MPTP induced Parkinson's disease C57Bl6 mice using a Morris water maze. I wanted to know if there are any specific dimensions to the MWM to be used. Most...

17 November 2020 4,558 3 View

How to select threshold for bi-variate frequency analysis of extreme rainfall events?

I am trying to do a bi-variate (average intensity and duration) frequency analysis of extreme rainfall events for urban flood studies. I have hourly rainfall data for 45 years and have delinted...

03 April 2019 8,282 3 View

What are the effects of Capacitor C3 in the circuit?

27 March 2017 8,840 18 View

How to assess platelet count manually in peripheral smear with only clumps?

how to assess platelet count manually in a peripheral smear with only clumps?

27 May 2016 8,301 3 View

How many days after foliar spray on plants , chlorophyll content can be estimated to compare before and after spray differences ?

in mustard crop ?

21 December 2015 2,689 10 View

What is the algorithm used by matlab's built-in 'bvp4c' function in solving boundary value problems?

I want to know the method used by 'bvp4c' to converge to the solution. Thanks.

04 August 2015 9,350 1 View

How much Electric power could be generated by a piezoelectric material by applying our weight on it?

Does this power generated can be stored in batteries? Application could be in shoes and foot paths in order to harvest energy. I'm working on these applications would like to know all the possible...

01 January 1970 8,428 4 View

Can linear DNA be used as template for site-directed mutagenesis?

Is it possible to induce site-directed substitution mutation by quick-change method on linear dsDNA? or it has to be cloned in some vector? If yes, should it be treated with the Dpn1 enzyme...

03 March 2021 401 4 View

Does anyone have a recommendation for yeast reporter gene plasmid/protocol?

Hi, could anyone recommend a plasmid and/or protocol for reporter gene assay in S. cerevisiae? I want to assess the effect of growth conditions on a transcription factor, so I want to clone it`s...

01 March 2021 210 1 View

Expression cloning by using cDNA,do I need to modify the cDNA first?

I am going to have a expression cloning of mammalian gene by using shuttle plasmid to transforming the E.coli However I don't know I should only inserting the Coding sequence ,or I can...

28 February 2021 5,440 3 View

Did anyone ever try using pLVX-TetOne Plasmid with a copGFP as a selection marker instead of Puromycin?

When using a lentiviral vector for inducible expression of genes using the Tet-On system. In the literature, I saw a widely used of pLVX-TetOne-Puro Plasmid, is there a reason why people prefer to...

25 February 2021 1,011 3 View

Difficulty in cloning of 1kb gene into vector?

I am facing difficulties in cloning a 1kb gene into a vector (pJIT163). I have my gene on interest (GOI) in pUC57 and want to clone in pJIT163 using SalI and BamHI restriction sites. I am getting...

25 February 2021 3,221 7 View

How to use protein A G beads to purify antibody?

Secretory universal influenza antibody CR9114 were successfully expressed by adenovirus vector ,then we used protein A G beads purify CR9114.But the molecular weight of the purified antibody under...

22 February 2021 4,318 2 View

Alternative to mediation analysis?

I am currently working on my dissertation and want to explore the role of competence as a mediator between the effect of colour of clothes on voting intention (focus on Western countries)....

21 February 2021 8,609 1 View

How COVID-19 has affected mental health?

The pandemic has led to depression and anxiety and its negative effects shouldn't be ignored. However, it has also led some people to become more resilient! What do you think? Thank you.

21 February 2021 4,733 19 View

Why are Sanger Sequencing reads interrupted in some edited clones?

I have a Crispr/Cas9 edited bulk population and I can briefly check the indel ratios in the bulk population using Sanger sequencing. After cloning single cells, I wanted to check the indel types...

18 February 2021 8,198 6 View

Hi, can anybody help me with the suggestions how can I amplify a stress induced gene of 1107 bp?

I am trying to amplify the TGA1 (Transcription factor 1 ) of Arabidopsis ,which is 1107 bp. I have treated the plants with Salycylic solution over night and extracted the RNA and make cDNA. I...

17 February 2021 7,802 2 View