How early can cognitive decline be detected in STZ induced type 1 diabetic rats?

13 November 2019 0 9K Report

I would like to know the least duration between confirmation of diabetes and detection of cognitive decline / impairment in streptozotocin induced type1 diabetic model rats

More Geetha Shivanna's questions See All

Can anyone help me in lentiviral vector cloning? i am facing problem after transformation. structural change observed?

i am trying to clone a lentiviral vector. After the transformation and purification, i see some structural changes where i am not able to digest the lentiviral plasmid with any enzyme. Can someone...

27 May 2024 2,165 3 View

Formula for sample size determination for creating in machine learning models for anomaly detection?

Is there any formula to find the sample size needed to create machine learning or deep learning models in the detection ,localization segmentation and classification of colon polyps

19 November 2023 1,134 2 View

Has anyone used a microtome for mice brain sections to study the hippocampal neurons,after Golgi stain??

I want to know if anyone has used a microtome to get sections of the mice brain to be stained with Golgi Cox for the study of the hippocampal neurons.

01 August 2023 7,866 1 View

Difficulty in debugging code to correct error - TypeError: '<' not supported ?

I am trying with a Unet code ontained from net ...in tensorflow. When trying to predict the segmentation result with the trained and saved model with metric " iou" using "CustomObjectScope"...

22 February 2021 5,673 1 View

Which Antibodies against AMPK and pAMPK do I use?

I'm trying to demonstrate that ghrelin activates AMPK in hippocampus and improves cognition in Parkinson's mice For the western blot which primary antibody should I use? For pAMPK...

21 January 2021 623 3 View

Is there a specific dimension of Morris water maze to assess the spatial memory in MPTP induced Parkinson's disease model of C57 Bl6 mice?

I am trying to assess spatial memory in MPTP induced Parkinson's disease C57Bl6 mice using a Morris water maze. I wanted to know if there are any specific dimensions to the MWM to be used. Most...

17 November 2020 4,865 3 View

Hi, to all friends from the field of ergonomic s and MSD studies, Posture assessment, please clarify my following queries?

1. When studies on posture and MSDs are conducted, is it mandatory to write the pain parts in medical terminologies like Thoracic, Lumbar region for report writing or can it be in simple terms as...

06 February 2020 8,428 6 View

Give possible suggestions to include unorganised food processing industry into the industrial scenario?

In the food processing industry many of it are unorganised one. In that case we couldn't take into account for analysis. Those firms are not comes under registered unit.

24 January 2020 7,760 2 View

In a hydrolytically degradable polymer, would two sterilization processes that caused the same Mw reduction cause the same change in degradation rate?

If I had a hydrolytically degradable polymer that went through two different sterilization processes and ended up with the same molecular weight reduction, would it degrade at the same rate in...

22 October 2019 2,913 0 View

FT IR results revealed the presence of three marked and well defined peaks in the finger print region,does it have any significance?

ft ir analysis results

19 June 2019 2,453 7 View

Can I multiplex a mouse monoclonal and rat primary for IHC (mouse brain tissue)?

Hi all, I was just wondering if anyone has experience with multiplexing a mouse monoclonal primary and a rat primary. I'm trying to multiplex by incubating them in the same well but was told by a...

06 August 2024 9,710 1 View

In what part of the brain´s rabbit bdnf is located and include references please?

I´ve been unable to find specific information about this neurotrophin in the CNS of rabbits exclusively. There is extensive info in mice, fish and rats, but in brain´s rabbit is hard to find....

04 August 2024 762 1 View

What can be the minimum number of animals (rats) to be used for a pilot/preliminary study on intradermal drug delivery?

Dear All I would like to know the minimum number of animals (rats) to be studied for a pilot/preliminary research on intradermal drug delivery. Can I proceed with 1 animal per group for a pilot work?

02 August 2024 4,570 1 View

CAD File of human's & rat's respiratory airways ?

Dear all, I am working on particle deposition in human's & rat's respiratory airways using CFD and I am looking for the 3D CAD file for my simulations (STEP or IGES format). If somone has such...

29 July 2024 1,092 2 View

Could GSH level be more in CCL4 rats than negative control rats?

i compare between the antioxidant and oxidative stress level between negative control rats (saline) and CCL4 intoxicated rats. i found the level of GSH and TAC is more in toxic group than normal....

26 July 2024 1,887 0 View

How can I calculate the SOD enzymatic activities using the spectrophotometer method?

I have to quantify the enzymatic activities of SOD in plants using a method that employs hypoxanthine-xanthine oxidase to create O2. The colorimetric reaction was performed by combining...

23 July 2024 8,492 3 View

Unexpected 35 kDa Band Observed During Expression and Purification of Dengue 3 NS5 RdRp Domain ?

Background: I am developing an RdRp assay for Dengue virus-3 and need to express and isolate the NS5 protein's RdRp domain (~101 kDa). My expression vector includes a GST tag followed by a 6x His...

13 July 2024 9,950 3 View

THP-1 cells completely dying with PMA, help?

Have been trying to induce THP-1 using PMA at different concentrations (range from 5 to 50 ng/mL) but after 48h all the conditions except the control are mostly dead, checked by trypan blue and...

03 July 2024 3,884 5 View

What could be the expression profile of alkaline phosphatase from lactic acid bacteria?

I have already cloned ALP gene of L rhamnosus into pET22b+ vector. Cloning is already confirmed by restriction digestion and sequencing. Cloning is achieved in BL21(DE3), Codon+, Rosetta and Lemo...

02 July 2024 5,332 3 View

What is the anti anaemic potentials of hibiscus sabdariffa on wister rats?

anti anaemic potentials if hibiscus sabdariffa

01 July 2024 8,429 0 View