Difficulty in debugging code to correct error - TypeError: '<' not supported ?

I am trying with a Unet code ontained from net ...in tensorflow. When trying to predict the segmentation result with the trained and saved model with metric " iou" using "CustomObjectScope"...

22 February 2021 5,254 1 View

Which Antibodies against AMPK and pAMPK do I use?

I'm trying to demonstrate that ghrelin activates AMPK in hippocampus and improves cognition in Parkinson's mice For the western blot which primary antibody should I use? For pAMPK...

21 January 2021 413 3 View

Is there a specific dimension of Morris water maze to assess the spatial memory in MPTP induced Parkinson's disease model of C57 Bl6 mice?

I am trying to assess spatial memory in MPTP induced Parkinson's disease C57Bl6 mice using a Morris water maze. I wanted to know if there are any specific dimensions to the MWM to be used. Most...

17 November 2020 4,558 3 View

Give possible suggestions to include unorganised food processing industry into the industrial scenario?

In the food processing industry many of it are unorganised one. In that case we couldn't take into account for analysis. Those firms are not comes under registered unit.

24 January 2020 7,565 2 View

FT IR results revealed the presence of three marked and well defined peaks in the finger print region,does it have any significance?

ft ir analysis results

19 June 2019 2,245 7 View

Does Fe/ Fe oxide nanoparticle precipitate out of solution?

I was trying to synthesise Fe/Fe oxide naoparticle through green rout ( plant mediated). On adding and stirring the plant extract to precursor the solution changed colour to dark red and fine...

31 August 2018 4,457 6 View

Can anyone recommend me the type of grid for TEM measurement of Fe3O4 silica nanoparticles and only silica nanoparticles?

22 June 2017 1,008 3 View

Will any bacteria show fluorescence on Nutrient Agar medium?

We have recently isolated a strain, which is a gram variant strain showing mucoidal colony morphology on nutrient agar plates and which glows (luminescence/ fluorescence) when viewed under UV...

12 March 2017 1,472 3 View

How to calculate percentage drug release?

04 October 2016 3,083 12 View

Is it possible to calculate area measurement for change detected of Multitemporal images

Hi all, Can any one suggest me how to calculate change Area measurement in change detection of multitemporal satellite images.

31 August 2016 4,397 7 View

Is there any test to determine the cardiovascular risk of patients with obesity? from the perspective of obesity?

We found tests to determine cardiovascular risk taking into account lipid profile, blood pressure, age, history of diabetes, other laboratories. But when weight is involved, there is a paradox. I...

02 March 2021 6,036 1 View

Why is the efficiency of viral mRNA electroporation in PBMC low?

Hello, I have been struggling with the electroporation of PBMC with HIV-I mRNA. Although the quality of the RNA generated is good, and the handling of the cells in meticulous, the frequency of...

27 February 2021 9,233 3 View

What are the pros and cons for mice versus rats?

Hi Everyone, I am designing a pilot study using rodents to assess the effects of simulated space environments (radiation + altered gravity) on microbiome and immune system. I have limited...

24 February 2021 1,825 3 View

What should be the duration of exposure to simulated space environments with Rodents?

Hi, I am currently working on a pilot study to get some data related to the impact of long duration spaceflight to the microbiome and the immune system. I plan to use rats and simulate...

22 February 2021 592 1 View

Do I need to introduce HFD to ob/ob mice to obtain a T2DM model?

I am planning to work on T2 diabetic animals and check the efficacy of probiotics. My initial plan is to fed B6 animals with HFD for a while. But now, I have the chance to work on ob/ob mice. My...

18 February 2021 6,896 3 View

How can you tell if your organotypic slice culture is healthy ?

I'm working on organotypic cerebellar slice cultures from postnatal rats and I'm not sure how to tell if the slices are healthy at any given point. I know there should be a lot of microglial...

15 February 2021 2,830 2 View

Why daily dexamethasone given to rats fed high fat/fructose/salt cause very high mortality rate?

rats were given daily dexamethasone in drinking water corresponding to 0.5 mg/kg or 2 mg/kg. These rats are simultaneously fed 25% fat, 3% salt in diet and 10% fructose in drinking water but...

15 February 2021 7,634 2 View

Can anyone suggest a way to dissolve insulin which is for rat/mouse injection?

We are trying to inject insulin to rat or mouse, but the insulin we've got is powder, and there are many ways for dissolve, like in saline, PBS, DMSO, or in Tris-HCl, can anyone give us a...

13 February 2021 8,426 10 View

Troubleshooting with immunofluoresence/confocal microscope? What went wrong?

Hi guys, I have no experience and little knowledge on immunofluorescence and confocal microscopy. I tried to visualize the expression of FXR in my cells (THP-1 macrophages), yet I did not...

11 February 2021 562 6 View

How do I fix tissue rolling while cutting frozen rat brain section with a cryostat?

I am cutting rat brain sections at 20um at -20C. The tissue is cutting nicely out of the O.C.T solution, but I can not get a decent section to adhere to the slide. I have included an image.

09 February 2021 1,345 5 View