I want to know if anyone has used a microtome to get sections of the mice brain to be stained with Golgi Cox for the study of the hippocampal neurons.
Geetha Shivanna I am using the microtome for acute brain slices and specifically in the hippocampus area. However I do not stain my samples. Do you have a specific question?
I am trying with a Unet code ontained from net ...in tensorflow. When trying to predict the segmentation result with the trained and saved model with metric " iou" using "CustomObjectScope"...
22 February 2021 5,254 1 View
I'm trying to demonstrate that ghrelin activates AMPK in hippocampus and improves cognition in Parkinson's mice For the western blot which primary antibody should I use? For pAMPK...
21 January 2021 413 3 View
I am trying to assess spatial memory in MPTP induced Parkinson's disease C57Bl6 mice using a Morris water maze. I wanted to know if there are any specific dimensions to the MWM to be used. Most...
17 November 2020 4,558 3 View
In the food processing industry many of it are unorganised one. In that case we couldn't take into account for analysis. Those firms are not comes under registered unit.
24 January 2020 7,565 2 View
ft ir analysis results
19 June 2019 2,245 7 View
I was trying to synthesise Fe/Fe oxide naoparticle through green rout ( plant mediated). On adding and stirring the plant extract to precursor the solution changed colour to dark red and fine...
31 August 2018 4,457 6 View
22 June 2017 1,008 3 View
We have recently isolated a strain, which is a gram variant strain showing mucoidal colony morphology on nutrient agar plates and which glows (luminescence/ fluorescence) when viewed under UV...
12 March 2017 1,472 3 View
04 October 2016 3,083 12 View
Hi all, Can any one suggest me how to calculate change Area measurement in change detection of multitemporal satellite images.
31 August 2016 4,397 7 View
Instead of checking only the mRNA level, I want to check the active protein level of MMP-1 in Liver tissue from mice. How can I do that?
03 March 2021 1,763 2 View
I have selected brain tumor images ...but now found that already lots of research done n this topic.
03 March 2021 5,774 3 View
I made a vertical section plot with WOA .nc file on ODV, and now I want to plot my stations (csv. file) in it. Does anyone know how to import my points to the section? They are two different...
01 March 2021 3,610 1 View
We have been using Thermo Fisher anti-cardiac Troponin T antibody for immunofluorescence staining in FFPE mouse heart sections but it was recently discontinued. The literature shows it to be the...
01 March 2021 4,937 1 View
There are different kinds of food stuffs which we intake daily according to our requirements. Carbohydrate, protein, fat, mineral, vitamin, water are mainly working for the growth and development...
01 March 2021 831 2 View
Can anyone help me choose primary antibodies for mouse liver hepatocytes and also in conjunction Cd8 cells. Also Cd3 cells. I want to look at hepatocytes and adjacent immune cells in mice with...
01 March 2021 6,953 1 View
Recently, we have injected s.c. B16F10 tumor cells in BL/6 mice. At around 12-15th day some animals showed ulcerations in tumors , with a big hole in the middle (it looks like a volcano). We have...
01 March 2021 9,938 3 View
Hello, I am writing a python script to generate my model and write the input file. The problem is I have 1000 elements in my mesh and I want to assign different material properties to each...
01 March 2021 9,511 3 View
Interested to stain mice brain tissue with WGA 488 conjugate, appreciate if anyone willing to offer me some guidance or suggestions! Thank you!
28 February 2021 3,951 2 View
I have two groups of brain samples, control and treated for example. It was total RNA nova seq sequencing. I tried all the available pipeline like: star+rsem+deseq2, Hista+stringtie+cuffdiff,...
27 February 2021 356 6 View