I want to know if anyone has used a microtome to get sections of the mice brain to be stained with Golgi Cox for the study of the hippocampal neurons.
Geetha Shivanna I am using the microtome for acute brain slices and specifically in the hippocampus area. However I do not stain my samples. Do you have a specific question?
i am trying to clone a lentiviral vector. After the transformation and purification, i see some structural changes where i am not able to digest the lentiviral plasmid with any enzyme. Can someone...
27 May 2024 2,165 3 View
Is there any formula to find the sample size needed to create machine learning or deep learning models in the detection ,localization segmentation and classification of colon polyps
19 November 2023 1,134 2 View
I am trying with a Unet code ontained from net ...in tensorflow. When trying to predict the segmentation result with the trained and saved model with metric " iou" using "CustomObjectScope"...
22 February 2021 5,673 1 View
I'm trying to demonstrate that ghrelin activates AMPK in hippocampus and improves cognition in Parkinson's mice For the western blot which primary antibody should I use? For pAMPK...
21 January 2021 623 3 View
I am trying to assess spatial memory in MPTP induced Parkinson's disease C57Bl6 mice using a Morris water maze. I wanted to know if there are any specific dimensions to the MWM to be used. Most...
17 November 2020 4,865 3 View
1. When studies on posture and MSDs are conducted, is it mandatory to write the pain parts in medical terminologies like Thoracic, Lumbar region for report writing or can it be in simple terms as...
06 February 2020 8,428 6 View
In the food processing industry many of it are unorganised one. In that case we couldn't take into account for analysis. Those firms are not comes under registered unit.
24 January 2020 7,760 2 View
I would like to know the least duration between confirmation of diabetes and detection of cognitive decline / impairment in streptozotocin induced type1 diabetic model rats
12 November 2019 9,375 0 View
If I had a hydrolytically degradable polymer that went through two different sterilization processes and ended up with the same molecular weight reduction, would it degrade at the same rate in...
22 October 2019 2,913 0 View
ft ir analysis results
19 June 2019 2,453 7 View
Hello, I am currently having problems with RNA extraction. I am using mouse liver (C57BL6J), and I have extracted RNA from mouse liver before. Before this experiment, my final RNA pellets were...
11 August 2024 7,082 3 View
Can anyone share any reference that gives mouse specific CD3 antibody sequence. I have been searching for this since few days and no luck. Thank you very much
08 August 2024 9,157 4 View
Vertebrates and invertebrates (e.g., arthropods: insects, crustaceans, etc.) have two different neuro-muscular systems given their differential construction: the former have an endoskeleton and...
07 August 2024 648 0 View
I am puzzled about the properties of gelatin, when used for tissue-embedding. Hard gelatin can be melt again by temperatures about 40°C. Formalin-fixed gelatin is like crosslinked protein and...
07 August 2024 1,686 1 View
I have modelled a steel structure using beam elements in Abaqus and attached to this structure reinforced concrete slab. The analysis that I am making is heat transfer of the structure. The...
07 August 2024 1,028 0 View
Hi, we have measured tryptic peptides using both DDA and DIA method on QExactive. In DDA replicates i saw unusual intensity drops occurring at the same sections of chromatograms in DDA replicates...
07 August 2024 3,218 4 View
Hello everyone, I have recently started using the microtome device for sectioning of paraffin-embedded mice lung. While I had some success in sectioning and observing proper ribbons, some of my...
06 August 2024 666 4 View
Hi all, I was just wondering if anyone has experience with multiplexing a mouse monoclonal primary and a rat primary. I'm trying to multiplex by incubating them in the same well but was told by a...
06 August 2024 9,710 1 View
i have sorted anti-NP specific plasma cells from bone marrow of C57BL/6 mice at certain times after immunization with variable counts and isolated total RNA using TRIZOL method for RT-PCR using...
05 August 2024 8,835 1 View
Brain and body mass together are positively correlated with lifespan (Hofman 1993). The duration of neural development is one of the best predictors of brain size, and conception is the best...
05 August 2024 6,247 3 View