Which among the following cell lines is best to be used for titrating anti-mouse EpCAM antibody by Flow cytometry?
1. Caco-2
2. DLD-1
3. HCT-116
4. SW480
I need to block the migration of macrophages into brain following a TMEV, virus infection in mice. The best way to follow is to use the mouse CCR2 inhibitors. Please suggest the most common and...
06 July 2018 6,722 0 View
I need to analyze the TNF-a production from mouse macrophages from brain by Flow cytometry. I see there are different protocols used by people. All I see is the addition of Brefeldin A to the...
05 June 2018 1,456 6 View
What are the suitable markers for distinguishing activated macrophages from activated microglia in mice brains by Flow cytometry?
04 May 2018 4,519 0 View
I want to set up the co-culture experiment using OT-II CD+T cells and splenic DC subsets for checking the CD4+ T cell activation. I was wondering out of the various splenic DC subsets, which DC...
06 July 2016 9,770 2 View
I want to check the activation status of CD8+ T cells from mouse spleen. I was wondering which activation markers I should use. Will CD44 and CD62 L together work to differentiate the naive CD8+ T...
06 July 2016 1,048 4 View
I wanted to know which marker I should use to sort the splenic macrophages from mice by flow cytometry? Actually I checked the literature and observed that people use CD11b or F4/80 as the marker...
05 June 2016 1,873 3 View
I have to sort splenic DCs from mice and was wondering which antibody is best to sort splenic DCs. Can I use just mouse anti- CD11c antibody for sorting splenic DCs as CD11c+ cells. Also, how many...
03 April 2016 3,501 4 View
I want to know whether it is a good idea to measure the IFN-g release by intracellular staining (Flow cytometry) from the in vitro culture of naive splenocytes infected with a specific M....
10 November 2015 9,697 9 View
I usually look at microglia isolated from mice brain using the standard percol gradient method without using Collagenase/DNAse steps i.e. just meshing the brain with 10 ml syringe plunger and then...
10 November 2015 7,578 6 View
I used Zombie aqua dye (fixable viability dye) for the first time to stain splenocytes by Flow cytometry. I mixed the live cells with heat killed cells (heated at 65 degrees for 1 min) in 1:1...
05 June 2015 7,530 5 View
I have carried out MFC experiments on three different volumes, 50, 500 and 1000 mL of wastewater. Results after MFC treatment shows that TDS and EC are more in larger volumes of water i.e. TDS and...
09 August 2024 9,621 0 View
Hello everyone! I observed in my culture (htert-RPE1 cells) an orange- red particle at the bottom of the dish. It is visible to the naked eye as a very very small red dot. Could it be a...
09 August 2024 2,824 3 View
I am working in fungal fermentation of soybean meal and there is bacterial growth in them at times. I am trying to quantify fungal cell counts and bacterial cells; but I haven't been able to do at...
07 August 2024 7,535 4 View
I would like to understand potential safety concerns while handling SEB in the lab. Especially while working in animal house facility. Would like to know precautions for handling. Sigma MSDS...
07 August 2024 6,034 3 View
Hi, I have a question about normalizing the MTT OD values for doing the statistical analysis. So, if we have 3 different plates and we call them 3 different replicates, so, first we would...
07 August 2024 8,106 4 View
Previously when I co-coluture anti-CD19(FMC63) CAR-Jurkat with Raji with E:T=5:1, Jurkat can eliminate Raji in 24h. However, when I test another CAR construct, although I can dectect totally CD69...
06 August 2024 641 2 View
After immunohistochemistry of previously fixed in PFA and EtOH and then frozen 20 μm sections of zebrafish brain, DAPI staining is very weak (right) compared to the same sections stained without...
05 August 2024 9,637 2 View
Are there any statistical methods to justify your sampling technique using SPSS or AMOS?
05 August 2024 9,153 4 View
I have tried several times to isolate lymphocytes from mouse spleen, but all attempts have been unsuccessful. I tried most available protocols. I used different dissociation media (HBSS with Ca...
04 August 2024 9,913 7 View
I have protein-membrane simulations (PDB, PSF, DCD) and have noticed that water molecules near the protein are not visible in the simulations. How can I fix this issue? Is there a way to place the...
04 August 2024 1,200 2 View