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Questions related from Tabasum Sidiq
I need to block the migration of macrophages into brain following a TMEV, virus infection in mice. The best way to follow is to use the mouse CCR2 inhibitors. Please suggest the most common and...
07 July 2018 6,679 0 View
I need to analyze the TNF-a production from mouse macrophages from brain by Flow cytometry. I see there are different protocols used by people. All I see is the addition of Brefeldin A to the...
06 June 2018 1,420 6 View
What are the suitable markers for distinguishing activated macrophages from activated microglia in mice brains by Flow cytometry?
05 May 2018 4,478 0 View
I want to check the activation status of CD8+ T cells from mouse spleen. I was wondering which activation markers I should use. Will CD44 and CD62 L together work to differentiate the naive CD8+ T...
07 July 2016 1,011 4 View
I want to set up the co-culture experiment using OT-II CD+T cells and splenic DC subsets for checking the CD4+ T cell activation. I was wondering out of the various splenic DC subsets, which DC...
07 July 2016 9,727 2 View
I wanted to know which marker I should use to sort the splenic macrophages from mice by flow cytometry? Actually I checked the literature and observed that people use CD11b or F4/80 as the marker...
06 June 2016 1,837 3 View
I have to sort splenic DCs from mice and was wondering which antibody is best to sort splenic DCs. Can I use just mouse anti- CD11c antibody for sorting splenic DCs as CD11c+ cells. Also, how many...
04 April 2016 3,472 4 View
I want to know whether it is a good idea to measure the IFN-g release by intracellular staining (Flow cytometry) from the in vitro culture of naive splenocytes infected with a specific M....
11 November 2015 9,666 9 View
I usually look at microglia isolated from mice brain using the standard percol gradient method without using Collagenase/DNAse steps i.e. just meshing the brain with 10 ml syringe plunger and then...
11 November 2015 7,537 6 View
I used Zombie aqua dye (fixable viability dye) for the first time to stain splenocytes by Flow cytometry. I mixed the live cells with heat killed cells (heated at 65 degrees for 1 min) in 1:1...
06 June 2015 7,471 5 View
I am doing mixed lymphocyte reaction for testing the reaction of C57BL/6 splenocytes to Balb/c splenocytes. I cultured the whole splenocytes of Balb/c mice with the irradiated splenocytes of...
05 May 2015 3,491 6 View
I have to culture splenocytes and retrogenic TB10.4 CD8 T cell clone separately. I was wondering if I should add sodium pyruvate to the RPMI 1640 medium. I usually add 1 mM sodium pyruvate to the...
04 April 2015 440 5 View
I need to check for the MLR (Mixed lymphocyte reaction) for the particular cells using MTT assay. I have to co-culture the responder cells (CD8 T cells) from one mouse strain with the stimulator...
03 March 2015 6,983 15 View
I need to treat bone marrow derived macrophages with a specific peptide for studying the antigen presentation. In the same experiment, I need to measure intracellular cytokine release and do CFSE...
02 February 2015 3,929 3 View
I need to culture retrogenic Ag specific CD8 cell clone with splenocytes in presence of an Ag (Class I dependent peptide) and check the proliferation and IFN-gamma expression by flow cytometry....
02 February 2015 3,875 4 View
I am facing the problem in culturing H.hepaticus on 5% Trypt Soy Agar plates. I successfully cultured it in the past. Using the same strategy, now I am not able to trouble shoot the problem even...
01 January 2015 5,044 4 View
I was wondering if anybody has any experience of the durability of Pertussis toxin used for EAE induction in mice. Once it is reconstituted, how long can we use it? Is it feasible to use it fresh...
01 January 2015 4,110 15 View
Which among the following cell lines is best to be used for titrating anti-mouse EpCAM antibody by Flow cytometry? 1. Caco-22. DLD-13. HCT-1164. SW480
11 November 2014 4,544 0 View
I need to stimulate NKT cells for the measurement of IFN-gamma release from these cells by flow cytometry. Can I use a stimulant other than alpha D-galcer to stimulate these cells for this purpose?
10 October 2014 1,112 11 View
Flow Cytometry
09 September 2014 9,074 12 View
Murine thymic epithelial cell isolation.
09 September 2014 7,036 3 View
Can somebody suggest which murine cells should be used for titrating CD107 (LAMP1) antibodies for Flow Cytometry? My idea is that we can use irradiated splenocytes as APCs and stimulate these...
09 September 2014 10,029 11 View
Has anybody irradiated C57/BL6 mice at 400 Rad for adoptive transfer of EAE? In the first attempt, I irradiated C57/BL6 mice at 350 Rad and I could not observe successful induction of disease...
09 September 2014 1,500 13 View
I usually get two populations of cells, with two peaks in histograms whenever I stain mice splenocytes with this antibody. Can anybody share his experience with me if using this antibody?
08 August 2014 2,878 8 View
I tried to stain splenocytes with cocktail containing FITC CD3 and APC NK1.1, but did not get the feasible results. Please suggest the appropriate marker for detecting NKTs in mice splenocytes.
08 August 2014 8,453 6 View
